{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:23:49Z","timestamp":1762100629229},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,14]],"date-time":"2006-07-14T00:00:00Z","timestamp":1152835200000},"content-version":"vor","delay-in-days":3969,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Uterine leiomyomas are benign tumors that arise clonally from smooth muscle cells of the myometrium. Cytogenetic studies of uterine leiomyomas have shown that about 40% have chromosome abnormalities and that deletion of 7q is a common finding. The observations suggest the possible location of a growth\u2010suppressor gene within the 7q21\u2010q22 region. Molecular genetic analysis of cytogenetically normal tumors has frequently shown somatic loss of specific tumor suppressor genes detected by loss of heterozygosity in the critical region. To test the hypothesis that chromosome region 7q21\u2010q22 contains a growth\u2010suppressor gene involved in the development of leiomyomas, we examined 92 leiomyomas for allelic loss of 7q markers spanning the cytogenetically defined critical region. Forty tumors with cytogenetically defined 7q deletion, 45 tumors without cytogenetically visible 7q deletion, and seven tumors with no cytogenetic information were examined for allelic loss of loci D7S489, D7S440, D7S492, D7S518, D7S471, D7S466, and D7S530. Loss of heterozygosity for one or more of these loci was observed in 23 of 40 (57.5%) of the tumors with deletion of 7q and in 2 of 45 cases without a cytogenetically visible deletion. The tumors with cytogenetic deletion of 7q, but no loss of 7q21\u2010q22 markers, were mosaics, with only a minority of cells containing the cytogenetic deletion. The critical region of loss is defined by the markers D7S518 and D7S471, each showing loss in approximately 50% of informative cases. These markers define a 10 cM region of 7q21\u2010q22 that is consistent with the cytogenetically defined smallest region of overlap and exclude loss of the <jats:italic>MET<\/jats:italic> oncogene locus and <jats:italic>WNT1<\/jats:italic>, the murine mammary tumor\u2010virus integration site, from the critical region. Our results further define a region that is consistently lost in leiomyomas with cytogenetic deletion of chromosome arm 7q. This region may contain a tumor suppressor gene involved in the development of a subset of leiomyomas.<\/jats:p>","DOI":"10.1002\/gcc.2870140109","type":"journal-article","created":{"date-parts":[[2007,2,22]],"date-time":"2007-02-22T00:43:24Z","timestamp":1172105004000},"page":"51-55","source":"Crossref","is-referenced-by-count":38,"title":["Molecular and cytogenetic analysis of chromosome 7 in uterine leiomyomas"],"prefix":"10.1002","volume":"14","author":[{"given":"C. S.","family":"Ishwad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. E.","family":"Ferrell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Davare","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Meloni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. A.","family":"Sandberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U.","family":"Surti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(88)90261-0"},{"key":"e_1_2_1_3_1","first-page":"440","volume-title":"Comprehensive Gynecology","author":"Droegemueller W","year":"1987"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(88)90306-8"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(88)90305-6"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(91)90009-E"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(92)90263-8"},{"key":"e_1_2_1_8_1","volume-title":"Guidelines for Cancer Cytogenetics, Supplement to an International System for Human Cytogenetic Nomenclature","author":"ISCN","year":"1991"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(91)90124-D"},{"key":"e_1_2_1_10_1","first-page":"209","article-title":"Uterine leiomyomas: Cytogenetic and histologic profile","volume":"80","author":"Meloni AM","year":"1992","journal-title":"Obstet Gynecol"},{"key":"e_1_2_1_11_1","first-page":"605","article-title":"Characteristic chromosome abnormalities, including rearrangements of 6p, del(7q), + 12 and t(12;14) in 44 uterine leiomyomas","volume":"85","author":"Niebert M","year":"1990","journal-title":"Hum Genet"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(93)90045-N"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-4608(93)90195-R"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1126\/science.2448875","article-title":"Primer\u2010directed amplification of DNA with thermostable DNA polymerase","volume":"239","author":"Saiki RK","year":"1988","journal-title":"Science"}],"container-title":["Genes, Chromosomes and Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgcc.2870140109","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.2870140109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T22:42:43Z","timestamp":1698360163000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.2870140109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,9]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,9]]}},"alternative-id":["10.1002\/gcc.2870140109"],"URL":"https:\/\/doi.org\/10.1002\/gcc.2870140109","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,9]]}}}