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While lymphedema\u2010associated and post\u2010irradiation ASs are almost always driven by a high\u2010level amplification of <jats:italic>MYC<\/jats:italic> (8q24), sporadic ASs, including those of breast parenchymal origin, typically lack <jats:italic>MYC<\/jats:italic> amplification. Here, we report a case of sporadic breast <jats:italic>MYC<\/jats:italic>\u2010amplified AS in a 19\u2010year\u2010old female with no history of lymphedema or irradiation, who was referred to our hospital for an enlarging right breast mass. After needle biopsy, the patient underwent right mastectomy and axillary lymphadenectomy. Microscopically, atypical endothelial cells proliferated and formed well\u2010defined or slit\u2010like vascular channels that invaded and dissected the breast parenchymal fat, ducts, and lobules. In a limited area, the tumor cells showed solid sheet\u2010like proliferation with increased mitotic figures of 40 per 2\u2009mm<jats:sup>2<\/jats:sup> with a small area of necrosis. Immunohistochemical analysis revealed strong positivity for c\u2010Myc. Fluorescence in\u00a0situ hybridization (FISH) with <jats:italic>MYC<\/jats:italic> break\u2010apart probes showed a high\u2010level 5\u2032 single signal amplification. The patient was disease\u2010free 16\u2009months post\u2010surgery. Nanopore sequencing successfully detected not only a high\u2010level amplification of the 8q24 region, including <jats:italic>MYC<\/jats:italic>, but also multiple structural variants of the 8q24 region. In\u2010depth analysis revealed extrachromosomal circular DNA amplification including the <jats:italic>MYC<\/jats:italic> protein\u2010coding region and upstream region but not the downstream region. We also performed methylation classification using nanopore\u2010based methylation data to successfully categorize the tumor as AS. This case report highlights the potential utility of nanopore sequencing in the diagnosis of sarcomas.<\/jats:p>","DOI":"10.1002\/gcc.70004","type":"journal-article","created":{"date-parts":[[2024,11,7]],"date-time":"2024-11-07T12:05:09Z","timestamp":1730981109000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Sporadic Breast Angiosarcoma With <i>MYC<\/i> Amplification on Extrachromosomal Circular <scp>DNA<\/scp> Detected Using Nanopore Sequencing in an Adolescent Female"],"prefix":"10.1002","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6991-747X","authenticated-orcid":false,"given":"Naohiro","family":"Makise","sequence":"first","affiliation":[{"name":"Division of Surgical Pathology Chiba Cancer Center  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Lin","sequence":"additional","affiliation":[{"name":"Division of Cell Therapy Chiba Cancer Center  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Kageyama","sequence":"additional","affiliation":[{"name":"Division of Surgical Pathology Chiba Cancer Center  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariko","family":"Oikawa","sequence":"additional","affiliation":[{"name":"Division of Surgical Pathology Chiba Cancer Center  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Sugiyama","sequence":"additional","affiliation":[{"name":"Division of Surgical Pathology Chiba Cancer Center  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidetada","family":"Kawana","sequence":"additional","affiliation":[{"name":"Division of Surgical Pathology Chiba Cancer Center  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akinobu","family":"Araki","sequence":"additional","affiliation":[{"name":"Division of Surgical Pathology Chiba Cancer Center  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shouko","family":"Hayama","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery Chiba Cancer Center  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rikiya","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery Chiba Cancer Center  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyuki","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Chiba Cancer Center  Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroto","family":"Kamoda","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery Chiba Cancer Center  Chiba 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