{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T12:14:38Z","timestamp":1788437678414,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T00:00:00Z","timestamp":1752192000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes Chromosomes &amp;amp; Cancer"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Recurrent\n                    <jats:italic>KMT2A<\/jats:italic>\n                    and\n                    <jats:italic>YAP1<\/jats:italic>\n                    related fusions have recently been reported in various mesenchymal neoplasms of different histogenesis. First,\n                    <jats:italic>YAP1::KMT2A<\/jats:italic>\n                    fusions have been described in a subset of MUC4\u2010negative sclerosing epithelioid fibrosarcomas (SEF), while\n                    <jats:italic>VIM::KMT2A<\/jats:italic>\n                    fusions in a handful of cases associated with an undifferentiated spindle cell phenotype lacking stromal hyalinization. On the other hand,\n                    <jats:italic>YAP1<\/jats:italic>\n                    gene rearrangements have been reported in a wide spectrum of sarcomas, including vascular neoplasms such as epithelioid hemangioendothelioma (EHE). Despite these molecular advances, occasional challenges in classification may occur even if the pathognomonic fusion is identified. In this study, we report such a case of a soft tissue sarcoma displaying an unusual morphology and immunoprofile, which remained unclassified even after a\n                    <jats:italic>YAP1::KMT2A<\/jats:italic>\n                    fusion was detected. The lesion occurred in the left leg of a 65\u2010year\u2010old female and microscopically closely resembled a SEF, with epithelioid morphology organized in cords, nests, and sheets in a heavy hyalinized background. Focally, the cells showed cytoplasmic vacuoles with eosinophilic material, reminiscent of the \u201cblisters cells\u201d seen in EHE. Moreover, by immunohistochemistry (IHC), the tumor showed diffuse reactivity for vascular markers, including ERG, CD31, CD34, and D2\u201040, as well as for TFE3, while being negative for MUC4, CAMTA1, smooth\u2010muscle actin, desmin, S100 and keratins. Targeted RNA sequencing revealed a\n                    <jats:italic>YAP1::KMT2A<\/jats:italic>\n                    fusion. Based on this molecular result and the conflicting morphologic and IHC findings, a definitive distinction between a MUC4\u2010negative SEF and an EHE could not been established. To further subclassify the lesion, subsequent clustering analysis using RNAseq signature was performed against a vast group of sarcoma types on the same array. Results showed that the tumor was in close proximity to the SEF group, admixed together with the other\n                    <jats:italic>YAP1::KMT2A<\/jats:italic>\n                    MUC4 negative SEF sarcomas. This case is highly instructive, as it shows another application of RNA sequencing in clinical practice when discordant or uncertain results between pathologic findings and fusion type may occur. Indeed, RNAseq signature could help, in this context, to better classify the tumor as a\n                    <jats:italic>YAP1::KMT2A<\/jats:italic>\n                    sarcoma instead of a vascular tumor. Larger series are needed to evaluate the pathogenesis of these tumors and the relevance of vascular markers expression.\n                  <\/jats:p>","DOI":"10.1002\/gcc.70059","type":"journal-article","created":{"date-parts":[[2025,7,12]],"date-time":"2025-07-12T03:53:40Z","timestamp":1752292420000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>YAP1::KMT2A<\/i>\n                    \u2010Rearranged Sarcoma: Report of a New Case With Unusual Morphology and Immunohistochemical Features"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8614-7622","authenticated-orcid":false,"given":"Caterina","family":"Fumagalli","sequence":"first","affiliation":[{"name":"Department of Pathology Hospital de la Santa Creu i Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Orellana","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital de la Santa Creu i Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00edlvia","family":"Bagu\u00e9","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital de la Santa Creu i Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malena","family":"Ferr\u00e9","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital de la Santa Creu i Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital de la Santa Creu i Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lluis","family":"Catas\u00fas","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital de la Santa Creu i Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaume","family":"Llauger","sequence":"additional","affiliation":[{"name":"Department of Radiology Hospital de la Santa Creu i Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Peir\u00f3","sequence":"additional","affiliation":[{"name":"Department of Traumatology and Orthopedics Hospital de la Santa Creu i Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul Zamora","family":"Alarc\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery Hospital de la Santa Creu i Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarina","family":"Majercakova","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Hospital de la Santa Creu i Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal","family":"Ter\u00e9s","sequence":"additional","affiliation":[{"name":"Department of Oncology Hospital de la Santa Creu i Sant Pau  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0434-636X","authenticated-orcid":false,"given":"Marie","family":"Karanian\u2010Philippe","sequence":"additional","affiliation":[{"name":"Department of Pathology Centre L\u00e9on B\u00e9rard, Centre de Recherche en Canc\u00e9rologie de Lyon, INSERM U1052\u2014CNRS UMR5286, Universit\u00e9 de Lyon, Universit\u00e9 Claude Bernard Lyon 1  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4731-7817","authenticated-orcid":false,"given":"Franck","family":"Tirode","sequence":"additional","affiliation":[{"name":"Department of Pathology Centre L\u00e9on B\u00e9rard, Centre de Recherche en Canc\u00e9rologie de Lyon, INSERM U1052\u2014CNRS UMR5286, Universit\u00e9 de Lyon, Universit\u00e9 Claude Bernard Lyon 1  Lyon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9717-8205","authenticated-orcid":false,"given":"Cristina R.","family":"Antonescu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Memorial Sloan Kettering Cancer Center  New York New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001382"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010020\u20100582\u20104"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001423"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.5053"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.13926"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.23138"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2015.04.002"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI126185"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22073"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2013.176"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010022\u201001096\u20106"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.23026"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3929"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11899\u2010020\u201000582\u20105"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14246211"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428\u2010024\u201003995\u20102"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010020\u20100582\u20104"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010022\u201001023\u20109"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010020\u20100646\u20105"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22835"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379\u2010022\u201001078\u20108"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.modpat.2023.100103"}],"container-title":["Genes, 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Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gcc.70059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T09:52:09Z","timestamp":1753782729000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gcc.70059"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7]]},"references-count":22,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1002\/gcc.70059"],"URL":"https:\/\/doi.org\/10.1002\/gcc.70059","archive":["Portico"],"relation":{},"ISSN":["1045-2257","1098-2264"],"issn-type":[{"value":"1045-2257","type":"print"},{"value":"1098-2264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7]]},"assertion":[{"value":"2025-01-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication 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