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In this study, we validate a 107\u2010gene Nanostring assay informed by previously established machine learning models using a prospective cohort of gastric adenocarcinoma tumours and tumour\u2010organoid pairs. Thirty\u2010eight gastric adenocarcinoma specimens and twelve parent tumour\u2010tumour organoid pairs were assigned TCGA and TME subtypes using gene expression measured by our custom Nanostring gene set. Subtypes were validated using gold\u2010standard tests for Epstein\u2013Barr virus (EBV) and microsatellite instability (MSI). Molecular subtype scores were compared to known clinicopathologic characteristics. The correlation between dose\u2013response and molecular subtypes using an organoid drug assay and the Cancer Cell Line Encyclopedia (CCLE) was investigated. TCGA and TME subtypes were successfully applied to all specimens. The relationship of molecular subtype scores in our population compared to public cohorts was statistically identical for Lauren Class and Signet Ring status. Our method achieved 100% accuracy in labeling EBV and MSI subtypes. We identified 81.8% and 63.6% concordance between parent tumour\u2010tumour organoid pairs for TME and TCGA subtypes, respectively. No significant correlation was identified between dose response to chemotherapy and molecular subtype scores. Analysis of the CCLE identified promising personalized therapy candidates for each molecular subtype. Our 107\u2010gene Nanostring test successfully assigns TCGA and TME molecular subtypes to clinical tumour and tumour organoid samples for use in future study.<\/jats:p>","DOI":"10.1002\/gcc.70090","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T05:44:52Z","timestamp":1763012692000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A 107 Gene Nanostring Assay Effectively Translates the Cancer Genome Atlas, and Tumour Microenvironment Gastric Cancer Molecular Classification to a Patient\u2010Derived Organoid Model"],"prefix":"10.1002","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8243-6139","authenticated-orcid":false,"given":"D.","family":"Skubleny","sequence":"first","affiliation":[{"name":"Department of Surgery Faculty of Medicine and Dentistry, University of Alberta  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Purich","sequence":"additional","affiliation":[{"name":"Department of Surgery Faculty of Medicine and Dentistry, University of Alberta  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Surgery Faculty of Medicine and Dentistry, University of Alberta  Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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