{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T12:50:19Z","timestamp":1782391819028,"version":"3.54.5"},"reference-count":13,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genes Chromosomes &amp; Cancer"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Pulmonary spindle cell tumors are aggressive neoplasms with limited systemic treatment options, although a subset may harbor actionable genomic alterations. Because conventional fusion assays may miss rearrangements involving atypical or previously uncharacterized partners, we systematically investigated oncogenic fusions in pulmonary spindle cell tumors using anchored multiplex PCR\u2010based targeted RNA sequencing. Formalin\u2010fixed, paraffin\u2010embedded tumor samples from 11 surgically resected pulmonary spindle cell tumors, excluding metastatic sarcomas, were analyzed using the FusionPlex Sarcoma panel supplemented with custom primers for RET, NTRK1, NTRK2, and NRG1. Two tumors (18.2%) harbored\n                    <jats:italic>ALK<\/jats:italic>\n                    fusions, identified as\n                    <jats:italic>PPFIBP1<\/jats:italic>\n                    ::\n                    <jats:italic>ALK<\/jats:italic>\n                    and\n                    <jats:italic>SYCL3<\/jats:italic>\n                    ::\n                    <jats:italic>ALK<\/jats:italic>\n                    . Both tumors showed positive\n                    <jats:italic>ALK<\/jats:italic>\n                    immunohistochemical staining. Histologically, the\n                    <jats:italic>PPFIBP1::ALK\u2010positive<\/jats:italic>\n                    tumor was composed of spindle\u2010shaped cells with a layered architecture, whereas the\n                    <jats:italic>SYCL3::ALK\u2010positive<\/jats:italic>\n                    tumor showed relatively round cells arranged in clusters with collagenous stroma, highlighting the morphologic heterogeneity of\n                    <jats:italic>ALK<\/jats:italic>\n                    \u2010rearranged pulmonary spindle cell tumors. These findings expand the molecular spectrum of pulmonary spindle cell tumors and identify rare\n                    <jats:italic>ALK<\/jats:italic>\n                    fusion partners in this setting. Our results support the incorporation of\n                    <jats:italic>ALK<\/jats:italic>\n                    immunohistochemistry as a screening tool and demonstrate the utility of anchored multiplex PCR\u2010based RNA sequencing for the detection of therapeutically relevant fusions, particularly those with uncommon partners. This integrated approach may refine the diagnosis and support consideration of\n                    <jats:italic>ALK<\/jats:italic>\n                    \u2010directed therapy in these rare tumors.\n                  <\/jats:p>","DOI":"10.1002\/gcc.70146","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T10:32:51Z","timestamp":1780309971000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detecting Rare\n                    <scp>ALK<\/scp>\n                    Gene Fusions in Unclassified Spindle Cell Lung Tumors Using Anchored Multiplex\n                    <scp>PCR<\/scp>\n                    \/Targeted\n                    <scp>RNA<\/scp>\n                    Next\u2010Generation Sequencing"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3569-2978","authenticated-orcid":false,"given":"Katsuhiro","family":"Masago","sequence":"first","affiliation":[{"name":"Department of Pathology and Molecular Diagnostics Aichi Cancer Center Hospital  Nagoya Aichi Japan"},{"name":"Department of Thoracic Surgery Teikyo University Mizonokuchi Hospital  Kawasaki Kanagawa Japan"},{"name":"Division of Translational Oncoimmunology Aichi Cancer Center Research Institute  Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0502-1655","authenticated-orcid":false,"given":"Katsutoshi","family":"Seto","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery Teikyo University Mizonokuchi Hospital  Kawasaki Kanagawa Japan"},{"name":"Division of Translational Oncoimmunology Aichi Cancer Center Research Institute  Nagoya Aichi Japan"},{"name":"Department of Thoracic Surgery Aichi Cancer Center Hospital  Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiichi","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Diagnostics Aichi Cancer Center Hospital  Nagoya Aichi Japan"},{"name":"Department of Thoracic Surgery Teikyo University Mizonokuchi Hospital  Kawasaki Kanagawa Japan"},{"name":"Division of Translational Oncoimmunology Aichi Cancer Center Research Institute  Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuko","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Diagnostics Aichi Cancer Center Hospital  Nagoya Aichi Japan"},{"name":"Department of Thoracic Surgery Teikyo University Mizonokuchi Hospital  Kawasaki Kanagawa Japan"},{"name":"Division of Translational Oncoimmunology Aichi Cancer Center Research Institute  Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiro","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine Kobe Central Hospital  Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshitsugu","family":"Horio","sequence":"additional","affiliation":[{"name":"Department of Thoracic Oncology Aichi Cancer Center Hospital  Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirokazu","family":"Matsushita","sequence":"additional","affiliation":[{"name":"Division of Translational Oncoimmunology Aichi Cancer Center Research Institute  Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery Teikyo University Mizonokuchi Hospital  Kawasaki Kanagawa Japan"},{"name":"Division of Translational Oncoimmunology Aichi Cancer Center Research Institute  Nagoya Aichi Japan"},{"name":"Department of Thoracic Oncology Aichi Cancer Center Hospital  Nagoya Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2017.03.005"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2015.62.0674"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41698-021-00201-3"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1200\/PO.24.00038"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3729"},{"issue":"2","key":"e_1_2_8_7_1","first-page":"993","article-title":"Weak\u2010Evidence Fusion Candidates Detected by a FusionPlex Assay Using the Ion Torrent System","volume":"35","author":"Fujita S.","year":"2021","journal-title":"In Vivo (Athens, Greece)"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2016-0488"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2017.181"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtho.2020.12.006"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2022.40.16_suppl.8530"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2012.05.093"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-11-0063"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2022.09.022"}],"container-title":["Genes, Chromosomes and 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