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For patient screening and routine diagnostics, fluorescence in\u00a0situ hybridization (FISH) using a dual\u2010color break\u2010apart probe is considered a reliable alternative. However, the sensitivity and specificity of FISH for detecting\n                    <jats:italic>ZNF384\u2010r<\/jats:italic>\n                    have not been systematically evaluated. In this study, we assessed the performance of FISH by comparing its results with RNA\u2010seq data from the same patient cohort. We also performed single\u2010nucleotide polymorphism array analysis to identify copy\u2010number alterations indicative of\n                    <jats:italic>ZNF384<\/jats:italic>\n                    \u2010r and the respective partner genes. While FISH proved highly specific, its sensitivity was lower than anticipated, potentially leading to misdiagnosis in a substantial proportion of patients.\n                  <\/jats:p>","DOI":"10.1002\/gcc.70161","type":"journal-article","created":{"date-parts":[[2026,8,22]],"date-time":"2026-08-22T04:05:44Z","timestamp":1787371544000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pitfalls and Challenges in the Detection of\n                    <scp>\n                      <i>ZNF384<\/i>\n                    <\/scp>\n                    Rearrangements by Fluorescence in Situ Hybridization"],"prefix":"10.1002","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0548-541X","authenticated-orcid":false,"given":"Karin","family":"Nebral","sequence":"first","affiliation":[{"name":"Labdia Labordiagnostik GmbH  Vienna Austria"},{"name":"St. Anna Children's Cancer Research Institute (CCRI)  Vienna 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