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Pandemics at the scale of COVID\u201019 can cause a global shortage of proprietary commercial reagents and BSL\u20102 laboratories to safely perform testing. Therefore, alternative solutions are urgently needed to address these challenges. An open\u2010source method,\n                    <jats:styled-content>m<\/jats:styled-content>\n                    agnetic\u2010nanoparticle\u2010\n                    <jats:styled-content>a<\/jats:styled-content>\n                    ided\n                    <jats:styled-content>v<\/jats:styled-content>\n                    iral\n                    <jats:styled-content>R<\/jats:styled-content>\n                    NA\n                    <jats:styled-content>i<\/jats:styled-content>\n                    solation from\n                    <jats:styled-content>c<\/jats:styled-content>\n                    ontagious\n                    <jats:styled-content>s<\/jats:styled-content>\n                    amples (MAVRICS), built upon readily available reagents, and easily assembled in any basically equipped laboratory, is thus developed. The performance of MAVRICS is evaluated using validated pathogen detection assays and real\u2010world and contrived samples. Unlike conventional methods, MAVRICS works directly in samples inactivated in phenol\u2010chloroform (e.g., TRIzol), thus allowing infectious samples to be handled safely without biocontainment facilities. MAVRICS allows wastewater biomass immobilized on membranes to be directly inactivated and lysed in TRIzol followed by RNA extraction by magnetic nanoparticles, thereby greatly reducing biohazard risk and simplifying processing procedures. Using 39 COVID\u201019 patient samples and two wastewater samples, it is shown that MAVRICS rivals commercial kits in detection of SARS\u2010CoV\u20102, influenza viruses, and respiratory syncytial virus. Therefore, MAVRICS is safe, fast, and scalable. It is field\u2010deployable with minimal equipment requirements and could become an enabling technology for widespread testing and wastewater monitoring of diverse pathogens.\n                  <\/jats:p>","DOI":"10.1002\/gch2.202000068","type":"journal-article","created":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T04:07:52Z","timestamp":1613966872000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A Robust, Safe, and Scalable Magnetic Nanoparticle Workflow for RNA Extraction of Pathogens from Clinical and Wastewater Samples"],"prefix":"10.1002","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5019-4291","authenticated-orcid":false,"given":"Gerardo","family":"Ramos\u2010Mandujano","sequence":"first","affiliation":[{"name":"Biological and Environmental Sciences and Engineering Division (BESE) King Abdullah University of Science and Technology (KAUST)  Thuwal 23955\u20106900 Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahul","family":"Salunke","sequence":"additional","affiliation":[{"name":"Biological and Environmental Sciences and Engineering Division (BESE) King Abdullah University of Science and Technology (KAUST)  Thuwal 23955\u20106900 Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Mfarrej","sequence":"additional","affiliation":[{"name":"Biological and Environmental Sciences and Engineering Division (BESE) King Abdullah University of Science and Technology (KAUST)  Thuwal 23955\u20106900 Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andri Taruna","family":"Rachmadi","sequence":"additional","affiliation":[{"name":"Biological and Environmental Sciences and Engineering Division (BESE) King Abdullah University of Science and Technology (KAUST)  Thuwal 23955\u20106900 Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharif","family":"Hala","sequence":"additional","affiliation":[{"name":"Biological and Environmental Sciences and Engineering Division (BESE) King Abdullah University of Science and Technology (KAUST)  Thuwal 23955\u20106900 Kingdom of Saudi Arabia"},{"name":"King Abdullah International Medical Research Centre King Saud University for Health Sciences Ministry of National Guard Health Affairs  Jeddah 21859 Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinna","family":"Xu","sequence":"additional","affiliation":[{"name":"Biological and Environmental Sciences and Engineering Division (BESE) King Abdullah University of Science and Technology (KAUST)  Thuwal 23955\u20106900 Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fadwa S.","family":"Alofi","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department King Fahad Hospital  Almadinah Almunwarah 11525 Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Asim","family":"Khogeer","sequence":"additional","affiliation":[{"name":"Plan and Research Department General Directorate of Health Affairs Makkah Region Ministry of Health  Mecca 11176 Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anwar M.","family":"Hashem","sequence":"additional","affiliation":[{"name":"Vaccines and Immunotherapy Unit King Fahd Medical Research Center King Abdulaziz University  Jeddah 21859 Saudi Arabia"},{"name":"Department of Medical Microbiology and Parasitology Faculty of Medicine King Abdulaziz University  Jeddah 21859 Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naif A. M.","family":"Almontashiri","sequence":"additional","affiliation":[{"name":"College of Applied Medical Sciences Taibah University  Almadinah Almunwarah 71491 Saudi Arabia"},{"name":"Center for Genetics and Inherited Diseases Taibah University  Almadinah Almunwarah 71491 Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afrah","family":"Alsomali","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department  King Abdullah Medical Complex Jeddah 24246 Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Digambar B.","family":"Shinde","sequence":"additional","affiliation":[{"name":"Division of Physical Science and Engineering King Abdullah University of Science and Technology (KAUST)  Thuwal 23955\u20106900 Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samir","family":"Hamdan","sequence":"additional","affiliation":[{"name":"Biological and Environmental Sciences and Engineering Division (BESE) King Abdullah University of Science and Technology (KAUST)  Thuwal 23955\u20106900 Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei\u2010Ying","family":"Hong","sequence":"additional","affiliation":[{"name":"Biological and Environmental Sciences and Engineering Division (BESE) King Abdullah University of Science and Technology (KAUST)  Thuwal 23955\u20106900 Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnab","family":"Pain","sequence":"additional","affiliation":[{"name":"Biological and Environmental Sciences and Engineering Division (BESE) King Abdullah University of Science and Technology (KAUST)  Thuwal 23955\u20106900 Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0827-8907","authenticated-orcid":false,"given":"Mo","family":"Li","sequence":"additional","affiliation":[{"name":"Biological and Environmental Sciences and Engineering Division (BESE) King Abdullah University of Science and Technology (KAUST)  Thuwal 23955\u20106900 Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,22]]},"reference":[{"key":"e_1_2_10_1_1","unstructured":"Centers for Disease Control and Prevention Division of Viral Diseases https:\/\/www.fda.gov\/media\/134922\/download(accessed: July2020)."},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb9263"},{"key":"e_1_2_10_3_1","author":"Bi C.","year":"2020","journal-title":"medRxiv."},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.03.035"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2001191"},{"key":"e_1_2_10_6_1","first-page":"1843","volume":"323","author":"Wang W.","year":"2020","journal-title":"JAMA"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.estlett.0c00357"},{"key":"e_1_2_10_8_1","unstructured":"O. 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