{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T20:52:44Z","timestamp":1790455964465,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T00:00:00Z","timestamp":1679270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000781","name":"European Research Council","doi-asserted-by":"publisher","award":["614787"],"award-info":[{"award-number":["614787"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/S003339\/1"],"award-info":[{"award-number":["EP\/S003339\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/V046594\/1"],"award-info":[{"award-number":["EP\/V046594\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","award":["EP\/K027263\/1"],"award-info":[{"award-number":["EP\/K027263\/1"]}],"id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Global Challenges"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Virus recognition has been driven to the forefront of molecular recognition research due to the COVID\u201019 pandemic. Development of highly sensitive recognition elements, both natural and synthetic is critical to facing such a global issue. However, as viruses mutate, it is possible for their recognition to wane through changes in the target substrate, which can lead to detection avoidance and increased false negatives. Likewise, the ability to detect specific variants is of great interest for clinical analysis of all viruses. Here, a hybrid aptamer\u2010molecularly imprinted polymer (aptaMIP), that maintains selective recognition for the spike protein template across various mutations, while improving performance over individual aptamer or MIP components (which themselves demonstrate excellent performance). The aptaMIP exhibits an equilibrium dissociation constant of 1.61 nM toward its template which matches or exceeds published examples of imprinting of the spike protein. The work here demonstrates that \u201cfixing\u201d the aptamer within a polymeric scaffold increases its capability to selectivity recognize its original target and points toward a methodology that will allow variant selective molecular recognition with exceptional affinity.<\/jats:p>","DOI":"10.1002\/gch2.202200215","type":"journal-article","created":{"date-parts":[[2023,3,20]],"date-time":"2023-03-20T09:41:47Z","timestamp":1679305307000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Highly Selective Aptamer\u2010Molecularly Imprinted Polymer Hybrids for Recognition of SARS\u2010CoV\u20102 Spike Protein Variants"],"prefix":"10.1002","volume":"7","author":[{"given":"Mark V.","family":"Sullivan","sequence":"first","affiliation":[{"name":"Leicester School of Pharmacy De Montfort University  The Gateway Leicester LE1 9BH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francia","family":"Allabush","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering University of Birmingham  Edgbaston Birmingham B15 2TT UK"},{"name":"School of Chemistry University of Birmingham  Edgbaston Birmingham B15 2TT UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harriet","family":"Flynn","sequence":"additional","affiliation":[{"name":"The Aptamer Group Windmill House Innovation Way  Heslington York, YO10 5BR UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Banushan","family":"Balansethupathy","sequence":"additional","affiliation":[{"name":"The Aptamer Group Windmill House Innovation Way  Heslington York, YO10 5BR UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph A.","family":"Reed","sequence":"additional","affiliation":[{"name":"The Aptamer Group Windmill House Innovation Way  Heslington York, YO10 5BR UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward T.","family":"Barnes","sequence":"additional","affiliation":[{"name":"The Aptamer Group Windmill House Innovation Way  Heslington York, YO10 5BR UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Callum","family":"Robson","sequence":"additional","affiliation":[{"name":"The Aptamer Group Windmill House Innovation Way  Heslington York, YO10 5BR UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phoebe","family":"O'Hara","sequence":"additional","affiliation":[{"name":"The Aptamer Group Windmill House Innovation Way  Heslington York, YO10 5BR UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura J.","family":"Milburn","sequence":"additional","affiliation":[{"name":"The Aptamer Group Windmill House Innovation Way  Heslington York, YO10 5BR UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Bunka","sequence":"additional","affiliation":[{"name":"The Aptamer Group Windmill House Innovation Way  Heslington York, YO10 5BR UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arron","family":"Tolley","sequence":"additional","affiliation":[{"name":"The Aptamer Group Windmill House Innovation Way  Heslington York, YO10 5BR UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula M.","family":"Mendes","sequence":"additional","affiliation":[{"name":"School of Chemical Engineering University of Birmingham  Edgbaston Birmingham B15 2TT UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James H. R.","family":"Tucker","sequence":"additional","affiliation":[{"name":"School of Chemistry University of Birmingham  Edgbaston Birmingham B15 2TT UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9380-5291","authenticated-orcid":false,"given":"Nicholas W.","family":"Turner","sequence":"additional","affiliation":[{"name":"Leicester School of Pharmacy De Montfort University  The Gateway Leicester LE1 9BH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,20]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2012-7"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113029"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00865-9"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2180-5"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1729069"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb2507"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00573-0"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.07.012"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.27577"},{"key":"e_1_2_9_10_1","unstructured":"Centers for Disease Control and Prevention https:\/\/www.cdc.gov\/coronavirus\/2019-ncov\/variants\/variant-classifications.html(accessed: April2022)."},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2020-0554"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.puhe.2020.06.006"},{"key":"e_1_2_9_13_1","unstructured":"FDA U.S Food & Drug Administration https:\/\/www.fda.gov\/medical-devices\/coronavirus-covid-19-and-medical-devices\/sars-cov-2-viral-mutations-impact-covid-19-tests(accessed: October2022)."},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202008663"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22084093"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmr.760"},{"key":"e_1_2_9_17_1","first-page":"1","volume-title":"Polymer Chemistry Series","author":"Canfarotta F.","year":"2018"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2016.030"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemmater.7b01936"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2021.122878"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.08.043"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00604-018-3123-9"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1PY00607J"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201404235"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5BM00341E"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.202100002"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matlet.2021.130898"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1SC04502D"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2021.131160"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/jfb11020043"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1NR03727G"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssensors.2c00100"},{"key":"e_1_2_9_33_1","unstructured":"MIP Discovery COVID\u201019 nanoMIP https:\/\/mipdiscovery.com\/covid19-nanomip(accessed: October2022)."},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1039\/C9NA00327D"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-022-04012-8"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1039\/D2TB00270A"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/nano10020306"},{"key":"e_1_2_9_38_1","first-page":"269","volume-title":"Methods in Molecular Biology","author":"Bossi A. M.","year":"2021"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2090"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1080\/14728222.2017.1271415"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.03.026"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(21)00170-5"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03402-9"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-021-00542-x"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2019.00115"},{"key":"e_1_2_9_46_1","first-page":"183","volume-title":"Methods in Molecular Biology","author":"Canfarotta F.","year":"2020"}],"container-title":["Global Challenges"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gch2.202200215","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/gch2.202200215","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gch2.202200215","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T16:17:58Z","timestamp":1692548278000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gch2.202200215"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,20]]},"references-count":46,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1002\/gch2.202200215"],"URL":"https:\/\/doi.org\/10.1002\/gch2.202200215","archive":["Portico"],"relation":{},"ISSN":["2056-6646","2056-6646"],"issn-type":[{"value":"2056-6646","type":"print"},{"value":"2056-6646","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,20]]},"assertion":[{"value":"2022-11-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-03-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"2200215"}}