{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:45:24Z","timestamp":1791049524832,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,6,25]],"date-time":"2002-06-25T00:00:00Z","timestamp":1024963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Summary: In addition to an essential role in chondrogenesis, <jats:italic>SOX9<\/jats:italic> is a highly conserved and integral part of the testis determining pathway in human and mouse. To determine whether <jats:italic>SOX9<\/jats:italic> is involved in sex determination in noneutherian mammals we cloned a marsupial orthologue and studied its expression. The tammar wallaby <jats:italic>SOX9<\/jats:italic> gene proved to be highly conserved, and maps to a region of the tammar genome syntenic to human chromosome 17. Marsupial <jats:italic>SOX9<\/jats:italic> transcripts were detected by RT\u2010PCR in the developing limb buds and both the developing ovary and testis from the first sign of gonadal development through to adulthood. Northern blot, in situ hybridisation, and immunohistochemical analyses showed that <jats:italic>SOX9<\/jats:italic> reaches high levels of expression in the developing testis, where it is confined to the Sertoli cell nuclei, and the brain. This is similar to the expression pattern seen in human and mouse embryos and is consistent with a conserved role for <jats:italic>SOX9<\/jats:italic> in vertebrate brain, skeletal, and gonadal development. In addition, <jats:italic>SOX9<\/jats:italic> was expressed in the developing scrotum and mammary gland primordium regions of the tammar up to the time of birth. SOX9 protein was also detected in the developing Wolffian duct epithelium in the male mesonephros. These previously undescribed locations of SOX9 expression suggest that SOX9 may play additional roles in the differentiation of the marsupial reproductive system. genesis 33:131\u2013139, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gene.10096","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T11:46:55Z","timestamp":1033904815000},"page":"131-139","source":"Crossref","is-referenced-by-count":24,"title":["SOX9 has both conserved and novel roles in marsupial sexual differentiation"],"prefix":"10.1002","volume":"33","author":[{"given":"Andrew J.","family":"Pask","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny L.","family":"Harry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer A. Marshall","family":"Graves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel J. Waugh","family":"O'Neill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon L.","family":"Layfield","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey","family":"Shaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marilyn B.","family":"Renfree","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,6,25]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81527-5"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2001.6648"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0697-174"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.111092198"},{"key":"e_1_2_1_6_1","volume-title":"Sex chromosomes and sex determining genes","author":"Cooper DW","year":"1993"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(200003)217:3<293::AID-DVDY7>3.0.CO;2-P"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/359531a0"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/372525a0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00307-X"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1195-347"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199905)215:1<69::AID-DVDY8>3.0.CO;2-N"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1320150103"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-8628(19991203)87:4<349::AID-AJMG13>3.0.CO;2-N"},{"key":"e_1_2_1_15_1","volume-title":"Sex chromosomes and sex determining genes","author":"Hughes RL","year":"1993"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1242\/dev.104.4.549","article-title":"M\u00fcllerian inhibiting substance production and testicular migration and decent in the pouch young of a marsupial","volume":"104","author":"Hutson JM","year":"1998","journal-title":"Development"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.9.2813"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD9950713"},{"key":"e_1_2_1_19_1","first-page":"1028","article-title":"Mutations in SOX9, the gene responsible for campomelic dysplasia and autosomal sex reversal","volume":"57","author":"Kwok C","year":"1995","journal-title":"Am J Hum Genet"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9625"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0996-62"},{"key":"e_1_2_1_22_1","volume-title":"Australian Code of Practise for the Care and Use of Animals for Scientific Purposes","author":"National Health and Medical Research Council","year":"1990"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/331716a0"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199808)212:4<522::AID-AJA5>3.0.CO;2-J"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.3.741"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/1526-968X(200008)27:4<145::AID-GENE30>3.0.CO;2-7"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.230424497"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1086\/316932"},{"key":"e_1_2_1_29_1","unstructured":"PooleWE SimmsNG WoodJT LubulwaM.1991.Tables for age determination of the Kangaroo Island wallaby (tammar) Macropus eugenii from body measurements. 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