{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:53:45Z","timestamp":1791273225412,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,3,8]],"date-time":"2001-03-08T00:00:00Z","timestamp":984009600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2001,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Summary: A GFP transgene has been integrated on the proximal part of the mouse X chromosome just distal of <jats:italic>Timp and Syn1.<\/jats:italic> During development, this X\u2010linked GFP transgene exhibits widespread green fluorescence throughout the embryonic and adult life of male mice but displays mosaic expression in tissues as a result of X\u2010inactivation in females. In living female embryos, inactivation of the transgene is imprinted in extraembryonic regions and random in the embryo proper, demonstrating that this reporter is behaving in a similar fashion to the majority of X\u2010linked loci, and so provides a vital readout of X chromosome activity. This is observation is further supported in T16H\/X female mice harboring the GFP transgene on the normal X chromosome where reporter inactivation is observed in somatic cells. The differential expression of GFP activity facilitates fluorescence activated cell sorting for the purification of GFP+ vs. GFP\u2010 cells from female embryonic tissues, thereby allowing access to populations of cells that have kept active a particular X chromosome. By tracking the activity of this X\u2010linked GFP transgene, we discovered that the primary and secondary giant cells of the X\/X placenta maintain an active paternal copy of this transgene on the presumed silenced paternal X\u2010chromosome. This finding implies that the imprint on the paternal X chromosome may be relaxed in these trophectodermal derivatives. genesis 29:133\u2013140, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gene.1016","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:44:55Z","timestamp":1030311895000},"page":"133-140","source":"Crossref","is-referenced-by-count":110,"title":["An X\u2010linked GFP transgene reveals unexpected paternal X\u2010chromosome activity in trophoblastic giant cells of the mouse placenta"],"prefix":"10.1002","volume":"29","author":[{"given":"Anna\u2010Katerina","family":"Hadjantonakis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liza L.","family":"Cox","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick P.L.","family":"Tam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andras","family":"Nagy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,3,8]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90519-I"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8303295"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2000.tb00907.x"},{"key":"e_1_2_1_5_1","first-page":"349","article-title":"Clonal analysis of X\u2010chromosome inactivation and the origin of the germline in the mouse embryo","volume":"88","author":"Gardner RL","year":"1985","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(98)00093-8"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/893"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/1526-968X(200007)27:3<95::AID-GENE10>3.0.CO;2-A"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004180000233"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.14.9120"},{"key":"e_1_2_1_11_1","volume-title":"Manipulating the mouse embryo. A laboratory manual","author":"Hogan BLM","year":"1994"},{"key":"e_1_2_1_12_1","first-page":"29","volume":"95","author":"Lyon MF","year":"1997","journal-title":"Mouse genome"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/293589a0"},{"key":"e_1_2_1_14_1","first-page":"53","article-title":"X\u2010chromosome activity in preimplantation mouse embryos from XX and XO mothers","volume":"46","author":"Monk M","year":"1978","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(90)90514-U"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90434-D"},{"key":"e_1_2_1_17_1","first-page":"1","article-title":"Non\u2010random X\u2010chromosome inactivation in mouse X\u2010autosome translocation embryos\u2014location of the inactivation centre","volume":"78","author":"Rastan S","year":"1983","journal-title":"J Embryol Exp Morphol"},{"key":"e_1_2_1_18_1","first-page":"379","article-title":"X\u2010chromosome deletions in embryo derived (EK) cell lines associated with lack of X\u2010chromosome inactivation","volume":"90","author":"Rastan S","year":"1985","journal-title":"J Embryol Exp Morph"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(86)90325-8"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9823"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(93)25013-R"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1242\/dev.115.3.703","article-title":"The somitogenetic potential of cells in the primitive streak and the tail bud of the organogenesis\u2010stage mouse embryo","volume":"115","author":"Tam PP","year":"1992","journal-title":"Development"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.121.4.1029"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0293-170"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgene.1016","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gene.1016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T15:23:06Z","timestamp":1700407386000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gene.1016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,3]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2001,3]]}},"alternative-id":["10.1002\/gene.1016"],"URL":"https:\/\/doi.org\/10.1002\/gene.1016","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,3]]}}}