{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T13:46:33Z","timestamp":1791899193784,"version":"4.3.4"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,11,7]],"date-time":"2002-11-07T00:00:00Z","timestamp":1036627200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Summary:\n                    <jats:italic>Cre<\/jats:italic>\n                    transgenic mice can be used to delete gene sequences flanked by\n                    <jats:italic>loxP<\/jats:italic>\n                    sites in specific somatic tissues. We have generated\n                    <jats:italic>vavCre<\/jats:italic>\n                    transgenic mice, which can be used to inactivate genes specifically in adult hematopoietic and endothelial cells. In these animals, a\n                    <jats:italic>Cre<\/jats:italic>\n                    transgene is expressed under control of murine\n                    <jats:italic>vav<\/jats:italic>\n                    gene regulatory elements. To assess their usefulness,\n                    <jats:italic>vavCre<\/jats:italic>\n                    transgenic mice were bred with\n                    <jats:italic>R26R<\/jats:italic>\n                    mice, which express a\n                    <jats:italic>lacZ<\/jats:italic>\n                    reporter gene only in cells where Cre\u2010mediated recombination has occurred.\n                    <jats:italic>VavCre\/R26R<\/jats:italic>\n                    double\u2010heterozygous offspring were analyzed by \u03b2\u2010galactosidase histochemistry and flow cytometry.\n                    <jats:italic>VavCre<\/jats:italic>\n                    \u2010mediated recombination occurred in most hematopoietic cells of all hematopoietic organs, including the hematopoietic progenitor\u2010rich bone marrow. Recombination also occurred in most endothelial and germ cells, but only rarely in other cell types. The recombination in both hematopoietic and endothelial lineages may partly reflect their putative shared ontogeny and provides a unique tool for simultaneous pan\u2010hematopoietic and endothelial mutagenesis. genesis 34:251\u2013256, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/gene.10161","type":"journal-article","created":{"date-parts":[[2002,11,15]],"date-time":"2002-11-15T10:56:35Z","timestamp":1037357795000},"page":"251-256","source":"Crossref","is-referenced-by-count":214,"title":["<i>vavCre<\/i>\n                    Transgenic mice: A tool for mutagenesis in hematopoietic and endothelial lineages"],"prefix":"10.1002","volume":"34","author":[{"given":"Pantelis","family":"Georgiades","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Ogilvy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Duval","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana R.","family":"Licence","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Stephen","family":"Charnock\u2010Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen K.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristin G.","family":"Print","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,11,7]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"297","article-title":"Developmental expression of the vav protooncogene","volume":"4","author":"Bustelo XR","year":"1993","journal-title":"Cell Growth Diff"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.125.4.725"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008942828960"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.26.12070"},{"key":"e_1_2_1_6_1","volume-title":"Manipulating the mouse embryo","author":"Hogan B","year":"1994"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.7.2248"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00328-2"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1101-1194"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1526-968X(200002)26:2<99::AID-GENE1>3.0.CO;2-B"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.2.419"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.6.1855"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.26.14943"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"4788","DOI":"10.4049\/jimmunol.163.9.4788","article-title":"Emergence of T, B, and myeloid lineage\u2010committed as well as multipotent hemopoietic progenitors in the aorta\u2010gonad\u2010mesonephros region of day 10 fetuses of the mouse","volume":"163","author":"Ohmura K","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202151"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1200878"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.6.1317"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1046-2023(05)80067-2"},{"key":"e_1_2_1_19_1","first-page":"363","article-title":"Isolation and characterization of murine vav2, a member of the vav family of proto\u2010oncogenes","volume":"13","author":"Schuebel KE","year":"1996","journal-title":"Oncogene"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/5007"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199807)28:07<2159::AID-IMMU2159>3.0.CO;2-B"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb06969.x"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgene.10161","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gene.10161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T15:26:37Z","timestamp":1696605997000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gene.10161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,11,7]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,12]]}},"alternative-id":["10.1002\/gene.10161"],"URL":"https:\/\/doi.org\/10.1002\/gene.10161","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,11,7]]}}}