{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:34:13Z","timestamp":1790667253526,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,3,13]],"date-time":"2003-03-13T00:00:00Z","timestamp":1047513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Summary: During vertebrate development the gonad has two possible fates, the testis or the ovary. The choice between these fates is made by a variety of sex\u2010determining mechanisms, from the sex\u2010determining gene on the Y chromosome (<jats:italic>Sry<\/jats:italic>) in mammals, to nongenetic temperature\u2010dependent systems in many reptiles. Despite the differences in the mechanisms at the top of the sex\u2010determining cascade, the resulting morphology and many genes involved in early testis and ovarian development are common to most vertebrates, leading to the hypothesis that the underlying processes of sex determination are conserved. In this study, we examined the early steps of gonad development in the red\u2010eared slider turtle (<jats:italic>Trachemys scripta<\/jats:italic>), a species that uses the temperature of egg incubation to determine sex. A dramatic increase in cell proliferation was observed in the male gonad during the earliest stages of sex determination. Using the localization of Wilms' Tumor suppressor 1 (WT1), we determined that this proliferation increase occurred in a population that contained pre\u2010Sertoli cells. The proliferation of pre\u2010Sertoli cells has been documented during sex determination in both mice and alligators, suggesting that proliferation of this cell type has an important role in vertebrate testis organogenesis and the determination of male fate. genesis 35:193\u2013201, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gene.10176","type":"journal-article","created":{"date-parts":[[2004,10,24]],"date-time":"2004-10-24T00:58:06Z","timestamp":1098579486000},"page":"193-201","source":"Crossref","is-referenced-by-count":27,"title":["Colocalization of WT1 and cell proliferation reveals conserved mechanisms in temperature\u2010dependent sex determination"],"prefix":"10.1002","volume":"35","author":[{"given":"Jennifer","family":"Schmahl","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Humphrey H.","family":"Yao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Pierucci\u2010Alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanche","family":"Capel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,3,13]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2001.0438"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402180315"},{"key":"e_1_2_1_4_1","first-page":"17","volume-title":"Gonadal development and function","author":"Burgoyne P","year":"1993"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(12)80012-3"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1110"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000133656"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1999.7360"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00453-6"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/1.5.293"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300023715"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1998.9068"},{"key":"e_1_2_1_13_1","first-page":"1781","article-title":"The evolution of WT1 sequence and expression pattern in the vertebrates","volume":"11","author":"Kent J","year":"1995","journal-title":"Oncogene"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1526-968X(200003)26:3<174::AID-GENE2>3.0.CO;2-J"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/7.4.709"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/351117a0"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90515-R"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1136\/jmg.36.6.452","article-title":"Incomplete masculinisation of XX subjects carrying the SRY gene on an inactive X chromosome","volume":"36","author":"Kusz K","year":"1999","journal-title":"J Med Genet"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"635","DOI":"10.1242\/dev.109.3.635","article-title":"XY female mice resulting from a heritable mutation in the murine primary testis determining gene, Tdy","volume":"109","author":"Lovell\u2010Badge R","year":"1990","journal-title":"Development"},{"key":"e_1_2_1_20_1","first-page":"297","article-title":"The initial phases of testicular organogenesis in the rat. An electron microscopy study","volume":"69","author":"Magre S","year":"1980","journal-title":"Arch Anat Microsc Morphol Exp"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(06)00415-5"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/gcen.1997.6946"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/221446a0"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-1809.1986.tb01029.x"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5193(89)80039-6"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(19991101)284:6<705::AID-JEZ12>3.0.CO;2-1"},{"key":"e_1_2_1_27_1","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.1242\/dev.119.4.1329","article-title":"Unclear localization of the protein encoded by the Wilms' tumor gene WT1 in embryonic and adult tissues","volume":"119","author":"Mundlos S","year":"1993","journal-title":"Development"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/346279a0"},{"key":"e_1_2_1_29_1","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1242\/dev.112.1.265","article-title":"In situ analysis of fetal, prepuberal and adult XX\u2010XY chimaeric mouse testes: Sertoli cells are predominantly, but not exclusively, XY","volume":"112","author":"Palmer SJ","year":"1991","journal-title":"Development"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.5.8.1345"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051700308"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(19980801)281:5<400::AID-JEZ5>3.0.CO;2-S"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1163\/156853885X00218"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.127.1.65"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1177\/35.11.3116075"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(96)00143-6"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00304622"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402700107"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1119"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0305-0491(98)00053-4"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402700113"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-010X(19980801)281:5<417::AID-JEZ7>3.0.CO;2-R"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.3109\/07435800009048640"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1113"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(99)00466-7"},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402600311"},{"key":"e_1_2_1_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051250207"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgene.10176","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gene.10176","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T02:47:55Z","timestamp":1696646875000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gene.10176"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,3,13]]},"references-count":46,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,4]]}},"alternative-id":["10.1002\/gene.10176"],"URL":"https:\/\/doi.org\/10.1002\/gene.10176","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,3,13]]}}}