{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:16:03Z","timestamp":1762175763656},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,3,13]],"date-time":"2003-03-13T00:00:00Z","timestamp":1047513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Summary: Mouse <jats:italic>Nkx2\u20105<\/jats:italic> gene is essential for early heart development and it is regulated by a complex array of regulatory modules. In order to establish an efficient in vivo system for mapping the <jats:italic>Nkx2\u20105<\/jats:italic> genomic locus for regulatory regions, we developed improved homologous recombination technology for use in <jats:italic>Escherichia coli<\/jats:italic> and then knocked an IRES\u2010hrGFP reporter gene into <jats:italic>Nkx2\u20105<\/jats:italic> gene in a 120 kb <jats:italic>Nkx2\u20105<\/jats:italic> bacterial artificial chromosome (BAC) clone. We employed the recombination genes <jats:italic>red\u03b1 and red\u03b2<\/jats:italic> under the pBAD promoter, which was specifically induced by the addition of L\u2010arabinose. Recombination was selected for by our universal targeting cassette which conferred kanamycin resistance in bacterial cells and neomycin resistance in mammalian cells. Transgenic mouse lines generated from this modified BAC clone closely resembled the endogenous <jats:italic>Nkx2\u20105<\/jats:italic> expression in the heart, pylorus sphincter, and spleen, but expression was not detected in the tongue. <jats:italic>Nkx2\u20105<\/jats:italic> BAC\u2010GFP expression was copy number\u2010dependent and locus site\u2010independent. BAC transgenics using the GFP reporter offers an efficient model system to study gene expression and regulation. genesis 35:220\u2013226, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gene.10181","type":"journal-article","created":{"date-parts":[[2004,10,24]],"date-time":"2004-10-24T00:58:06Z","timestamp":1098579486000},"page":"220-226","source":"Crossref","is-referenced-by-count":15,"title":["Expression of <i>Nkx2<\/i>\u2010<i>5<\/i>\u2010GFP bacterial artificial chromosome transgenic mice closely resembles endogenous <i>Nkx2<\/i>\u2010<i>5<\/i> gene activity"],"prefix":"10.1002","volume":"35","author":[{"given":"Xuan","family":"Chi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu\u2010xing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco J.","family":"DeMayo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan M.","family":"Rosenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"Schwartz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,3,13]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.118.3.719"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/24.15.3118"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.128.10.1857"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.11.2205"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008858014473"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.2000.6451"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.1.75"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.119.3.969"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.13.1654"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.10022"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.6.1555"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300901"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.126.4.839"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5373.108"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.116.4.1021"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"4187","DOI":"10.1242\/dev.126.19.4187","article-title":"Building the heart piece by piece: modularity of cis\u2010elements regulating Nkx2\u20105 transcription","volume":"126","author":"Schwartz RJ","year":"1999","journal-title":"Development"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1242\/dev.126.6.1269","article-title":"The cardiac homeobox gene Csx\/Nkx2.5 lies genetically upstream of multiple genes essential for heart development","volume":"126","author":"Tanaka M","year":"1999","journal-title":"Development"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"1439","DOI":"10.1242\/dev.126.7.1439","article-title":"Complex modular cis\u2010acting elements regulate expression of the cardiac specifying homeobox gene Csx\/Nkx2.5","volume":"126","author":"Tanaka M","year":"1999","journal-title":"Development"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgene.10181","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gene.10181","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T02:47:58Z","timestamp":1696646878000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gene.10181"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,3,13]]},"references-count":18,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2003,4]]}},"alternative-id":["10.1002\/gene.10181"],"URL":"https:\/\/doi.org\/10.1002\/gene.10181","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,3,13]]}}}