{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T04:40:44Z","timestamp":1779338444624,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,8,6]],"date-time":"2001-08-06T00:00:00Z","timestamp":997056000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Summary: The mixed lineage leukemia gene (<jats:italic>MLL<\/jats:italic>) was originally identified through its involvement in reciprocal translocations in leukemias. <jats:italic>MLL<\/jats:italic> codes for a large multidomain protein and bears homology to the <jats:italic>Drosophila<\/jats:italic> developmental control gene trithorax in two small domains in the amino terminal region, the central zinc finger domain and the carboxy SET domain. Like the <jats:italic>Drosophila<\/jats:italic> trx, <jats:italic>MLL<\/jats:italic> has also been shown to be a positive regulator of <jats:italic>Hox<\/jats:italic> gene expression. We have targeted <jats:italic>Mll<\/jats:italic> (the murine homologue of <jats:italic>MLL<\/jats:italic>) in exon 5 causing expression of three truncated in\u2010frame <jats:italic>Mll<\/jats:italic> transcripts. These transcripts retain all or some of the AT hook motifs and the DMT domain. This mutant allele causes early in vivo preimplantation lethality of homozygous embryos prior to the 2\u2010cell stage. Embryos cultured in vitro progress to the 2\u2010cell stage, but further development is arrested. The heterozygotes exhibit mild skeletal defects as well as defects in some neuroectodermal derivatives. genesis 30:201\u2013212, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gene.1066","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T19:56:46Z","timestamp":1030305406000},"page":"201-212","source":"Crossref","is-referenced-by-count":61,"title":["Truncation of the <i>MLL<\/i> gene in exon 5 by gene targeting leads to early preimplantation lethality of homozygous embryos"],"prefix":"10.1002","volume":"30","author":[{"given":"Paul","family":"Ayton","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon F.","family":"Sneddon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald B.","family":"Palmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ian R.","family":"Rosewell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Owen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryan","family":"Young","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Presley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vasanta","family":"Subramanian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,8,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.45.28407"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870130202"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1242\/dev.117.1.119","article-title":"Trithorax regulates multiple homeotic genes in the bithorax and Antennapedia complexes and exerts different tissue specific, parasegment\u2010specific and promoter specific effects on each","volume":"117","author":"Breen TR","year":"1993","journal-title":"Development"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-85232-9_26"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.040574897"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1990.tb07875.x"},{"key":"e_1_2_1_8_1","first-page":"3811","article-title":"Cloning of All\u20101 the locus involved in leukaemias with t(4:11)(q21;q23) chromosome translocations","volume":"52","author":"Cimino G","year":"1991","journal-title":"Cancer Res"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1999.01439.x"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1092-113"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.5.843"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.16.7884"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1526-968X(200003)26:3<189::AID-GENE4>3.0.CO;2-5"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(97)80075-5"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1093\/genetics\/139.2.797","article-title":"Identification of Polycomb and trithorax group responsive elements in the regulatory regions of the Drosophila homeotic genes Sex combs reduced","volume":"139","author":"Gindhart JG Jr","year":"1995","journal-title":"Genetics"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V90.5.1799"},{"key":"e_1_2_1_17_1","first-page":"196","volume-title":"Manipulating the mouse embryo. A laboratory manual","author":"Hogan B","year":"1996"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-9525(93)90227-9"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.20.2478"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/385454a0"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0177(199805)212:1<38::AID-AJA4>3.0.CO;2-3"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.13.6350"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.10.4303"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.18.8424"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(96)00364-2"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(93)90084-4"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.10.4738"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1604321"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.26.12160"},{"key":"e_1_2_1_30_1","first-page":"1766","article-title":"Complete exon structure of the ALL\u20101 gene","volume":"56","author":"Rasio D","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_1_31_1","doi-asserted-by":"crossref","first-page":"8573","DOI":"10.1016\/S0021-9258(19)38926-4","article-title":"The AT\u2010DNA binding domain of mammalian high mobility groupI chromosomal proteins. A novel peptide motif for recognizing DNA structure","volume":"265","author":"Reeves R","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.23.6979"},{"key":"e_1_2_1_33_1","first-page":"531","volume-title":"Regulation of zygotic gene activation in the mouse","author":"Shultz RM","year":"1993"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.1.122"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.10.5976"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90602-9"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-60479-9_1"},{"key":"e_1_2_1_38_1","doi-asserted-by":"crossref","first-page":"2949","DOI":"10.1242\/dev.121.9.2949","article-title":"Temporally restricted spatial localization of acetylated isoforms of histone H4 and RNA polymerase II in the 2\u2010cell mouse embryo","volume":"121","author":"Worrad DM","year":"1995","journal-title":"Development"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.1.108.413k11_108_117"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.14.7286"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/378505a0"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.18.10632"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.22.10610"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgene.1066","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gene.1066","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T17:11:25Z","timestamp":1700413885000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gene.1066"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,8]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,8]]}},"alternative-id":["10.1002\/gene.1066"],"URL":"https:\/\/doi.org\/10.1002\/gene.1066","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,8]]}}}