{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T03:21:52Z","timestamp":1775186512481,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,7,23]],"date-time":"2004-07-23T00:00:00Z","timestamp":1090540800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Summary: The <jats:italic>Hnf4<\/jats:italic>\u03b1 gene belongs to a family of trancriptional regulators required for liver development and function. <jats:italic>Hnf4<\/jats:italic>\u03b1 is also expressed in other tissues, including the newly formed visceral endoderm of the early postimplantation embryo, and later in embryogenesis in the gut epithelium and the kidney. The regulatory sequences involved in controlling expression of <jats:italic>Hnf4<\/jats:italic>\u03b1 at these diverse sites are not clearly understood. Here we used homologous recombination to introduce Cre recombinase coding sequences into the endogenous <jats:italic>Hnf4<\/jats:italic>\u03b1 locus. Crossing <jats:italic>Hnf4<\/jats:italic>\u03b1<jats:sup><jats:italic>Creex2\/+<\/jats:italic><\/jats:sup> mice with <jats:italic>R26<\/jats:italic><jats:sup>R<\/jats:sup> partners allowed us to follow the pattern of Cre\u2010mediated recombination. Our results show that recombination of the reporter allele closely follows endogenous <jats:italic>Hnf4<\/jats:italic>\u03b1 expression, but with a slight temporal delay. Thus, the <jats:italic>Hnf4<\/jats:italic>\u03b1<jats:sup><jats:italic>Creex2<\/jats:italic><\/jats:sup> strain should prove useful for conditionally deleting gene activity in the liver, gut epithelium, or kidney. genesis 39:206\u2013211, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gene.20047","type":"journal-article","created":{"date-parts":[[2004,7,23]],"date-time":"2004-07-23T16:01:47Z","timestamp":1090598507000},"page":"206-211","source":"Crossref","is-referenced-by-count":13,"title":["Targeted insertion of an <i>IRES Cre<\/i> into the <i>Hnf4<\/i>\u03b1 locus: Cre\u2010mediated recombination in the liver, kidney, and gut epithelium"],"prefix":"10.1002","volume":"39","author":[{"given":"St\u00e9phane D.","family":"Vincent","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth J.","family":"Robertson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,7,23]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.241349398"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.8.20.2466"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0177(2000)9999:9999<::AID-DVDY1051>3.0.CO;2-N"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(02)00328-3"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.16.7598"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.124.2.279"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.12.6191"},{"key":"e_1_2_1_9_1","volume-title":"Manipulating the mouse embryo, a laboratory manual","author":"Hogan BL","year":"1994"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00052-0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)37059-X"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.2.497"},{"key":"e_1_2_1_13_1","volume-title":"Hepatocyte nuclear factor 4 alpha","author":"Sladek FM","year":"2001"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/5007"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0925-4773(94)90017-5"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(01)00521-4"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2795(199811)51:3<274::AID-MRD6>3.0.CO;2-M"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2003.50273"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.11.7276"}],"container-title":["genesis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fgene.20047","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/gene.20047","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T20:10:14Z","timestamp":1700251814000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/gene.20047"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,7]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2004,7]]}},"alternative-id":["10.1002\/gene.20047"],"URL":"https:\/\/doi.org\/10.1002\/gene.20047","archive":["Portico"],"relation":{},"ISSN":["1526-954X","1526-968X"],"issn-type":[{"value":"1526-954X","type":"print"},{"value":"1526-968X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,7]]}}}