{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:27:53Z","timestamp":1791278873147,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,7,27]],"date-time":"2004-07-27T00:00:00Z","timestamp":1090886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The function of most vertebrate genes remains unknown or uncertain. Insertional mutagenesis offers one approach to identify and understand the function of these genes. Transposons have been used successfully in lower organisms and plants for insertional mutagenesis, but until activation of the <jats:italic>Sleeping Beauty<\/jats:italic> (SB) transposon system, there was no indication of active DNA\u2010based transposons in vertebrates. Investigator\u2010driven insertional mutagenesis in vertebrates has relied on retroviral insertions or selection of low\u2010frequency integration of naked DNA in ES cell lines. We have combined the highly active SB transposon with gene\u2010trapping technology to demonstrate that transposon traps can be used for insertional mutagenesis screens in vertebrates. In our studies about one\u2010fourth of the trap insertions appear to be in transcriptional units, a rate that is commensurate with random integration. We show that gene\u2010traps coupled to a fluorescent protein reporter gene can be used to detect insertions into genes active in specific cells of living zebrafish embryos, supporting use of our transposon traps for high\u2010throughput functional genomic screens in vertebrates. genesis 39:225\u2013233, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/gene.20049","type":"journal-article","created":{"date-parts":[[2004,7,27]],"date-time":"2004-07-27T21:00:39Z","timestamp":1090962039000},"page":"225-233","source":"Crossref","is-referenced-by-count":52,"title":["Transposon vectors for gene\u2010trap insertional mutagenesis in vertebrates"],"prefix":"10.1002","volume":"39","author":[{"given":"Karl J.","family":"Clark","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aron M.","family":"Geurts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason B.","family":"Bell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Perry B.","family":"Hackett","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,7,27]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.24.3141"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1525-0016(03)00211-9"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/165.1.243"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)01779-8"},{"key":"e_1_2_1_6_1","unstructured":"ClarkKJ.2003.The development ofSleeping Beautytransposons for the insertional mutagenesis of vertebrates. 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