{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T13:12:20Z","timestamp":1783602740068,"version":"3.55.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T00:00:00Z","timestamp":1736899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T00:00:00Z","timestamp":1736899200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Genetic Epidemiology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Polyunsaturated fatty acids (PUFAs) including omega\u20103 and omega\u20106 are obtained from diet and can be measured objectively in plasma or red blood cells (RBCs) membrane biomarkers, representing different dietary exposure windows. In vivo conversion of omega\u20103 and omega\u20106 PUFAs from short\u2010 to long\u2010chain counterparts occurs via a shared metabolic pathway involving fatty acid desaturases and elongase. This analysis leveraged genome\u2010wide association study (GWAS) summary statistics for RBC and plasma PUFAs, along with expression quantitative trait loci (eQTL) to estimate tissue\u2010specific genetically predicted gene expression effects for delta\u20105 desaturase (\n                    <jats:italic>FADS1<\/jats:italic>\n                    ), delta\u20106 desaturase (\n                    <jats:italic>FADS2<\/jats:italic>\n                    ), and elongase (\n                    <jats:italic>ELOVL2<\/jats:italic>\n                    ) on changes in RBC and plasma biomarkers. Using colocalization, we identified shared variants associated with both increased gene expression and changes in RBC PUFA levels in relevant PUFA metabolism tissues (i.e., adipose, liver, muscle, and whole blood). We observed differences in RBC versus plasma PUFA levels for genetically predicted increase in\n                    <jats:italic>FADS1<\/jats:italic>\n                    and\n                    <jats:italic>FADS2<\/jats:italic>\n                    gene expression, primarily for omega\u20106 PUFAs linoleic acid (LA) and arachidonic acid (AA). The colocalization analysis identified rs102275 to be significantly associated with a 0.69% increase in total RBC membrane\u2010bound LA levels (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20095.4\u2009\u00d7\u200910\n                    <jats:sup>\u221212<\/jats:sup>\n                    ). Future PUFA genetic studies examining long\u2010term PUFA biomarkers are needed to confirm our results.\n                  <\/jats:p>","DOI":"10.1002\/gepi.22613","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T09:20:25Z","timestamp":1736932825000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Genetically Predicted Gene Expression Effects on Changes in Red Blood Cell and Plasma Polyunsaturated Fatty Acids"],"prefix":"10.1002","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8802-4431","authenticated-orcid":false,"given":"Nikhil K.","family":"Khankari","sequence":"first","affiliation":[{"name":"Division of Genetic Medicine, Department of Medicine Vanderbilt University Medical Center Nashville USA"},{"name":"Vanderbilt Genetics Institute Vanderbilt University Medical Center Nashville USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Su","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, Department of Medicine Vanderbilt University Medical Center Nashville USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuyin","family":"Cai","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, Department of Medicine Vanderbilt University Medical Center Nashville USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, Department of Medicine Vanderbilt University Medical Center Nashville USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Jasper","sequence":"additional","affiliation":[{"name":"Division of Quantitative Sciences, Department of Obstetrics and Gynecology Vanderbilt University Medical Center Nashville USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacklyn N.","family":"Hellwege","sequence":"additional","affiliation":[{"name":"Division of Genetic Medicine, Department of Medicine Vanderbilt University Medical Center Nashville USA"},{"name":"Vanderbilt Genetics Institute Vanderbilt University Medical Center Nashville USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harvey J.","family":"Murff","sequence":"additional","affiliation":[{"name":"Division of Geriatric Medicine, Department of Medicine Vanderbilt University Medical Center Nashville USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha J.","family":"Shrubsole","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, Department of Medicine Vanderbilt University Medical Center Nashville USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jirong","family":"Long","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, Department of Medicine Vanderbilt University Medical Center Nashville USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Todd L.","family":"Edwards","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, Department of Medicine Vanderbilt University Medical Center Nashville USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, Department of Medicine Vanderbilt University Medical Center Nashville 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