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A variety of genome\u2010wide methods have been developed, each leveraging different biochemical principles \u2014 from direct capture of Spo11\u2010oligonucleotide covalent intermediates to sequencing protein\u2010bound single\u2010stranded DNA (ssDNA), in situ DNA end labeling, and chromatin immunoprecipitation (ChIP). Direct approaches such as Spo11\u2010oligo mappin, CC\u2010seq and in situ DNA end labeling (END\u2010seq) achieve nucleotide\u2010resolution detection of the initial cleavage site, but typically require large amounts of starting material. ChIP ssDNA\u2010based methods (e.g., ChIP\u2010SSDS) enrich for recombinase associated proteins\u2010 bound (DMC1, RAD51, RPA) intermediates, reflecting an indirect proxy of DSB sites, and are limited to early resection stages. Together, while these complementary methods comprise a toolbox for dissecting meiotic hotspot landscapes, a more robust and facile approach for routine detection of meiotic DSB hotspots across diverse species remains to be developed.<\/jats:p>","DOI":"10.1002\/ggn2.202500019","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T07:07:52Z","timestamp":1784012872000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mapping Meiotic Recombination DNA Double\u2010Strand Breaks (DSBs) Hotspots \u2014Methodological Advances and Challenges"],"prefix":"10.1002","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1248-2687","authenticated-orcid":false,"given":"Jianqiang","family":"Bao","sequence":"first","affiliation":[{"name":"Key Laboratory of Reproductive Health Diseases Research and Translation NHC Key Laboratory of Tropical Disease Control Ministry of Education Hainan Medical 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