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In this study, we performed a detailed analysis of the expression of the Gi\/o\u2010coupled receptor P2Y<jats:sub>12<\/jats:sub> in the brain. Northern blot analysis demonstrated that P2Y<jats:sub>12<\/jats:sub> is expressed predominantly in the brain, and to a lesser extent in the spleen. The cellular localization of P2Y<jats:sub>12<\/jats:sub> was investigated by in situ hybridization, and P2Y<jats:sub>12<\/jats:sub> mRNA was detected in small cells distributed throughout the brain, including the hippocampus. Expression of P2Y<jats:sub>12<\/jats:sub> was also observed in naive and axotomized facial nuclei, and the number of P2Y<jats:sub>12<\/jats:sub>\u2010expressing cells increased following facial nerve axotomy. Selective expression of P2Y<jats:sub>12<\/jats:sub> mRNA in microglia was confirmed by double\u2010label in situ hybridization and immunohistochemistry with antibodies against NeuN and Iba1 as an immunohistochemical marker for neurons and microglia, respectively. Hardly any P2Y<jats:sub>12<\/jats:sub> mRNA was detected in macrophages obtained from the spleen and abdominal cavity, which share many surface molecules with microglia. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/glia.10293","type":"journal-article","created":{"date-parts":[[2003,11,3]],"date-time":"2003-11-03T08:01:42Z","timestamp":1067846502000},"page":"242-250","source":"Crossref","is-referenced-by-count":216,"title":["Selective expression of Gi\/o\u2010coupled ATP receptor P2Y<sub>12<\/sub> in microglia in rat brain"],"prefix":"10.1002","volume":"44","author":[{"given":"Yo","family":"Sasaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Hoshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chihiro","family":"Akazawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuko","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayami","family":"Tsuzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhide","family":"Inoue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Kohsaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,8,18]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.1106"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.156.4.1531"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.3.579"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10161"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2000.0750965.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/35051599"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-06-01975.2001"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10149"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10150"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701732"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.11.4106-4114.2000"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-2236(96)10049-7"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.116.1.201"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0168-0102(93)90047-T"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1992.tb11356.x"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1996.66062500.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701241"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.1994.tb00552.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2001.00514.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10154"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/84224"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0723n.x"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fglia.10293","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.10293","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T11:53:56Z","timestamp":1700222036000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.10293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,8,18]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2003,12]]}},"alternative-id":["10.1002\/glia.10293"],"URL":"https:\/\/doi.org\/10.1002\/glia.10293","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,8,18]]}}}