{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:44:46Z","timestamp":1790192686276,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,4,15]],"date-time":"2004-04-15T00:00:00Z","timestamp":1081987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006280","name":"Ministerio de Ciencia y Tecnolog\u00eda","doi-asserted-by":"crossref","award":["SAF2002-04016"],"award-info":[{"award-number":["SAF2002-04016"]}],"id":[{"id":"10.13039\/501100006280","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Acad\u00e8mia de Ci\u00e8ncies M\u00e8diques de Catalunya i Balears, Spain"},{"name":"EC","award":["IST-2001-35094"],"award-info":[{"award-number":["IST-2001-35094"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We assessed the effects of FK506 administration on regeneration after a 6\u2010mm gap repair with a collagen guide seeded with allogeneic Schwann cells (SCs) in the mouse sciatic nerve. SCs were isolated from predegenerated adult sciatic nerves and expanded in culture using a defined medium, before being seeded in the collagen guide embedded in Matrigel. Functional reinnervation was evaluated by noninvasive methods to determine recovery of motor, sensory, and autonomic functions in the hindpaw over 4 months postoperation. Histological analysis of the regenerated nerves was performed at the end of the study. Using simple collagen guides for tubulization repair, treatment with an immunosuppressant dose of FK506 (5 mg\/kg\/day) resulted in significant improvement of the onset and the degree of reinnervation. While the introduction of allogeneic SCs did not improve regeneration versus a collagen guide filled only with Matrigel, treatment with FK506 allowed for successful regeneration in all the mice and for significant improvement in the levels of functional recovery. Compared with the untreated group, there was greater survival of transplanted pre\u2010labeled SCs in the FK506\u2010treated animals. Morphologically, the best nerve regeneration (in terms of nerve caliber and numbers of myelinated axons) was obtained with SC\u2010seeded guides from FK506\u2010treated animals. Thus, FK506 should be considered as adjunct therapy for various types of tubulization repair. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/glia.20025","type":"journal-article","created":{"date-parts":[[2004,4,15]],"date-time":"2004-04-15T18:15:55Z","timestamp":1082052955000},"page":"120-129","source":"Crossref","is-referenced-by-count":62,"title":["FK506 enhances regeneration of axons across long peripheral nerve gaps repaired with collagen guides seeded with allogeneic Schwann cells"],"prefix":"10.1002","volume":"47","author":[{"given":"Esther","family":"Udina","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco 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