{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T02:49:48Z","timestamp":1761706188379},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,4,30]],"date-time":"2004-04-30T00:00:00Z","timestamp":1083283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>N\u2010myc downstream\u2010regulated gene 1 (NDRG1)\/RTP\/Drg1\/Cap43\/rit42\/TDD5\/Ndr1 is expressed ubiquitously and has been proposed to play a role in growth arrest and cell differentiation. A recent study showed that mutation of this gene is responsible for hereditary motor and sensory neuropathy\u2010Lom. However, the role of this gene in the peripheral nervous system is not fully understood. In our study, rabbit polyclonal antibodies were raised against this gene product and were used to examine changes in its expression over the time course of Wallerian degeneration and ensuing regeneration after crush injury of mouse sciatic nerves. Fluorescent immunohistochemistry showed that NDRG1 was expressed over the intact nerve fibers. Double labeling with a Schwann cell (SC) marker, S\u2010100 protein (S\u2010100), revealed that NDRG1 was localized in the cytoplasm of S\u2010100\u2010positive Schwann cells (SCs). NDRG1 expression was maintained in the early stage of myelin degradation but was then markedly depleted at the end stage of myelin degradation when frequent occurrence of BrdU\u2010labeled SCs was observed (at 7\u20139 days). The depletion of NDRG1 at this time point was also confirmed by Western blotting analysis. NDRG1 expression finally recovered at the stage of remyelination, with immunoreactivity stronger than that in intact nerves. These findings suggest that NDRG1 may play an important role in the terminal differentiation of SCs during nerve regeneration. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/glia.20037","type":"journal-article","created":{"date-parts":[[2004,4,30]],"date-time":"2004-04-30T16:03:14Z","timestamp":1083340994000},"page":"325-334","source":"Crossref","is-referenced-by-count":18,"title":["N\u2010myc downstream\u2010regulated gene 1 expression in injured sciatic nerves"],"prefix":"10.1002","volume":"47","author":[{"given":"Kazuho","family":"Hirata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuaki","family":"Masuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wataru","family":"Morikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian\u2010Wen","family":"He","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akio","family":"Kuraoka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michihiko","family":"Kuwano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaru","family":"Kawabuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,4,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.2833"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.2001.1055"},{"key":"e_1_2_6_4_1","first-page":"1731","article-title":"The Drg\u20101 gene suppresses tumor metastasis in prostate cancer","volume":"63","author":"Bandyopadhyay S","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_6_5_1","first-page":"161","volume-title":"Glial cell development","author":"Colman DR","year":"2001"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.13.030190.000355"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.1069"},{"key":"e_1_2_6_8_1","first-page":"749","article-title":"Drg\u20101 as a differentiation\u2010related, putative metastatic suppressor gene in human colon cancer","volume":"60","author":"Guan RJ","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.10108"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007012916530"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1460-9568.2000.00307.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10105"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-2236(98)01391-5"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1086\/302978"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)81173-3"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.47.29659"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-9452(97)00182-9"},{"key":"e_1_2_6_18_1","first-page":"4439","article-title":"Inhibition of tumor cell growth by RTP\/rit42 and its responsiveness to p53 and DNA damage","volume":"58","author":"Kurdistani SK","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-002-0460-9"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.10.4988"},{"key":"e_1_2_6_21_1","first-page":"5853","article-title":"Distribution of cells expressing myc proteins in human colorectal epithelium, polyps, and malignant tumors","volume":"52","author":"Melhem MF","year":"1992","journal-title":"Cancer Res"},{"key":"e_1_2_6_22_1","first-page":"2145","article-title":"High expression of the Cap43 gene in infiltrating macrophages of human renal cell carcinomas","volume":"7","author":"Nishie A","year":"2001","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_23_1","first-page":"744","article-title":"Electron microscopic study of Wallerian degeneration in cutaneous nerve caused by mechanical injury","volume":"17","author":"O'Daly JA","year":"1967","journal-title":"Lab Invest"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1780"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(99)00056-7"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2613.1997.280362.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1017934810825"},{"key":"e_1_2_6_28_1","first-page":"38","article-title":"Carcinogenic nickel induces genes involved with hypoxic stress","volume":"60","author":"Salnikow K","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a022051"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205992"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.10083"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(99)00025-8"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01187086"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00845-5"},{"key":"e_1_2_6_35_1","first-page":"85","article-title":"A novel gene which is up\u2010regulated during colon epithelial cell differentiation and down\u2010regulated in colorectal neoplasms","volume":"77","author":"van Belzen N","year":"1997","journal-title":"Lab Invest"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod61.3.681"},{"key":"e_1_2_6_37_1","first-page":"166","article-title":"Changes in the phosphorylation states of connexin43 in myoepithelial cells of lactating rat mammary glands","volume":"72","author":"Yamanaka I","year":"1997","journal-title":"Eur J Cell Biol"},{"key":"e_1_2_6_38_1","first-page":"2182","article-title":"Cap43, a novel gene specifically induced by Ni2+ compounds","volume":"58","author":"Zhou D","year":"1998","journal-title":"Cancer Res"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fglia.20037","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.20037","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T01:12:52Z","timestamp":1696641172000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.20037"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4,30]]},"references-count":37,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,9]]}},"alternative-id":["10.1002\/glia.20037"],"URL":"https:\/\/doi.org\/10.1002\/glia.20037","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,4,30]]}}}