{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T11:17:42Z","timestamp":1791890262795,"version":"4.3.4"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,6,16]],"date-time":"2004-06-16T00:00:00Z","timestamp":1087344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Reversible tyrosine phosphorylation is integral to the process of oligodendrocyte differentiation. To interfere with the subset of the phosphorylation cycle overseen by protein tyrosine phosphatase \u03f5 (PTP\u03f5) in oligodendrocytes, we applied a substrate\u2010trapping approach in the development of transgenic mice overexpressing a catalytically inactive, transmembrane PTP\u03f5\u2010hemaglutinin (tm\u2010PTP\u03f5\u2010HA) from the dual promoter element of the gene encoding the myelin protein 2\u2032,3\u2032\u2010cyclic nucleotide 3\u2032\u2010phosphodiesterase (CNP). Transgene expression peaked during the active myelinating period, at 2\u20133 weeks postnatal. Two tyrosine phosphoproteins, \u03b1\u2010enolase and \u03b2\u2010actin, were phosphorylated to a greater degree in transgenic mice. Despite a high degree of tm\u2010PTP\u03f5\u2010HA expression, myelin was grossly normal in nearly all axonal tracts. Phenotypic abnormalities were limited to optic nerve, where a decrease in the degree of myelination was reflected by reduced levels of myelin proteins on postnatal day 21 (PND21), as well as a decrease in the density of differentiated oligodendrocytes. The optic chiasm was reduced in thickness in transgenic mice; optic nerves similarly exhibited a reduction in transverse width. Further analyses of the optic pathway demonstrated that transgenic protein was unexpectedly present in retinal ganglion cells, whose axons are the targets of myelination by optic nerve oligodendrocytes. On PND28, transgenic protein declined dramatically in both oligodendrocytes and retinal ganglion cells contributing to the recovery of optic nerve myelination. Thus, delayed myelination arises only when tm\u2010PTP\u03f5\u2010HA is simultaneously expressed in myelin\u2010forming glia and their neuronal targets. While tm\u2010PTP\u03f5 related signaling pathways may figure in axon\u2010glial interactions, interfering with tm\u2010PTP\u03f5 activity does not perceptibly affect the development or myelinating capacity of most oligodendrocytes. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/glia.20078","type":"journal-article","created":{"date-parts":[[2004,6,16]],"date-time":"2004-06-16T21:02:53Z","timestamp":1087419773000},"page":"278-297","source":"Crossref","is-referenced-by-count":7,"title":["Expression of a catalytically inactive transmembrane protein tyrosine phosphatase \u03f5 (tm\u2010PTP\u03f5) delays optic nerve myelination"],"prefix":"10.1002","volume":"48","author":[{"given":"Naser","family":"Muja","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabor","family":"Lovas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Romm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dietrich","family":"Machleder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mukul","family":"Ranjan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vittorio","family":"Gallo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynn D.","family":"Hudson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,6,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3540581"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1999.721362.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(199606)17:2<169::AID-GLIA8>3.0.CO;2-Y"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/382555a0"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M205810200"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.19-02-00759.1999"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1076\/ejom.35.1.3.13057","article-title":"Myelinogenesis in the optic nerve of (C57BL \u00d7 CBA) F1 hybrid mice: a morphometric analysis","volume":"35","author":"Dangata YY","year":"1997","journal-title":"Eur J Morphol"},{"issue":"1","key":"e_1_2_6_9_1","first-page":"117","article-title":"Postnatal development of the optic nerve in (C57BL \u00d7 CBA)F1 hybrid mice: general changes in morphometric parameters","volume":"189","author":"Dangata YY","year":"1996","journal-title":"J Anat"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1996.66030936.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490240211"},{"key":"e_1_2_6_12_1","first-page":"533","article-title":"Muscle fiber conduction velocity calculated from EMG power spectra","volume":"25","author":"Eberstein A","year":"1985","journal-title":"Electromyogr Clin Neurophysiol"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.44.26116"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80730-8"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-001-0357-z"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1203790"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(83)90335-9"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4547(19980815)53:4<393::AID-JNR1>3.0.CO;2-1"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.20.7706-7715.2000"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1004"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1998.0707"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00016.2002"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1990.tb07523.x"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.1073"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4695(199904)39:1<81::AID-NEU7>3.0.CO;2-K"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)82237-2"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1136(20000215)29:4<376::AID-GLIA8>3.0.CO;2-S"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1080-8914(06)80039-6"},{"key":"e_1_2_6_29_1","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1046\/j.1471-4159.81.s1.15_2.x","article-title":"Transgenic mice exhibiting oligodendrocyte\u2010specific expression of a mutant tyrosine phosphatase epsilon","volume":"81","author":"Muja N","year":"2002","journal-title":"J Neurochem"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(97)00193-9"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1037\/h0029465"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0129"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80755-2"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.2.647"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.15.4036"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.1996.0029"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903510410"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490320203"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(94)90451-0"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-328X(98)00237-X"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001090050149"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(98)00295-X"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(94)00190-P"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(00)00179-3"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/370571a0"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcne.2000.0939"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a124743"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.10.3030"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(00)00196-4"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.2002.01127.x"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.1401"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10368"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5266.1328"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.5.1624"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fglia.20078","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.20078","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T22:57:58Z","timestamp":1696892278000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.20078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6,16]]},"references-count":53,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["10.1002\/glia.20078"],"URL":"https:\/\/doi.org\/10.1002\/glia.20078","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,6,16]]}}}