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We addressed this issue using physiological methods on cultured and acutely dissociated rat astrocytes. Prior work showed that the intracellular lactate\u2010induced acidification (LIA) of astrocytes is diminished by benzolamide, a poorly permeant, nonspecific CA inhibitor. We demonstrate that pretreatment of astrocytes with phosphatidylinositol\u2010specific phospholipase C (PI\u2010PLC) results in a similar inhibition of the mean LIA (by 66 \u00b1 3%), suggesting that the glycosylphosphatidylinositol\u2010anchored CA IV was responsible. Pretreatment of astrocytes with CA IV inhibitory antisera also markedly reduced the mean LIA in both cultured cortical (by 46 \u00b1 4%) and acutely dissociated hippocampal astrocytes (by 54 \u00b1 8%). Pre\u2010immune sera had no effect. The inhibition produced by PIPLC or CA IV antisera was not significantly less than that by benzolamide, suggesting that the majority of detectable surface CA activity was attributable to CA IV. Thus, our data collectively document the presence of CAIV on the surface of brain astrocytes, and suggest that this is the predominant CA isoform on these cells. \u00a9 2005 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/glia.20277","type":"journal-article","created":{"date-parts":[[2005,11,1]],"date-time":"2005-11-01T22:16:47Z","timestamp":1130883407000},"page":"241-247","source":"Crossref","is-referenced-by-count":42,"title":["Functional demonstration of surface carbonic anhydrase IV activity on rat astrocytes"],"prefix":"10.1002","volume":"53","author":[{"given":"Nataliya","family":"Svichar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Esquenazi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdul","family":"Waheed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William S.","family":"Sly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitchell","family":"Chesler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0353124"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.110.4.467"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1988.255.6.C844"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.48.30096"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.19.7457"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1992.67.1.29"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.16.7786"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-0082(90)90034-E"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00010.2003"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02473299"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-0082(95)00039-9"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:HIJO.0000023387.02793.af"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-003-0616-2"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1999.5938"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.15.6823"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3430281"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001756-199201000-00028"},{"key":"e_1_2_5_19_1","first-page":"26","article-title":"A simplified micromethod for the determination of carbonic anhydrase and its inhibitors","volume":"130","author":"Maren TH","year":"1960","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_5_20_1","first-page":"901","article-title":"Chemical properties of carbonic anhydrase IV, the membrane\u2010bound enzyme","volume":"44","author":"Maren TH","year":"1993","journal-title":"Mol Pharmacol"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90223-Z"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.22.15701"},{"key":"e_1_2_5_23_1","doi-asserted-by":"crossref","unstructured":"NagelhusEA MathiisenTM BatemanAC HaugF\u2010M OttersenOP GrubbJH WaheedA SlyWS.2005.Carbonic anhydrase XIV is enriched in specific membrane domains of retinal pigment epithelium M\u00fcller cells and astrocytes. 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