{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T05:54:15Z","timestamp":1760853255003},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,1,3]],"date-time":"2007-01-03T00:00:00Z","timestamp":1167782400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The use of anti\u2010neutrophil serum (anti\u2010PMN) to induce neutropenia has been assessed for neuroprotection, modulation of microgliosis and astrogliosis, effects on oxidative stress, and intactness of blood\u2010brain barrier (BBB) following injection of the excitotoxin quinolinic acid (QUIN) into rat striatum. At 1 day following QUIN injection, considerable striatal neurodegeneration was measured (Fluoro\u2010Jade B marker). At this time, marked microgliosis (OX\u201042 marker) and astrogliosis (GFAP marker) were evident in QUIN\u2010injected striatum. Treatment of QUIN\u2010injected animals with anti\u2010PMN protected neurons (48% reduction of striatal neuron loss) and inhibited microgliosis (61% reduction) and astrogliosis (43% reduction) compared with QUIN injection alone. Anti\u2010PMN treatment was effective in decreasing levels of superoxide anions (by 42%) compared with QUIN alone; in addition, expressions of the neutrophil enzyme myeloperoxidase and the neutrophil oxidant 3\u2010chlorotyrosine were markedly reduced (by 79 and 72%, respectively) with neutrophil depletion. QUIN\u2010induced leakiness in BBB was indicated by elevated striatal levels of the blood protein fibrinogen, a result confirmed using Evans blue dye; anti\u2010PMN was effective in reducing BBB permeability. Measurements from QUIN\u2010injected animals directly confirmed anti\u2010PMN efficacy in diminishing numbers of circulating neutrophils. Longer term neuroprotection and reduced microgliosis were also observed at 7 days post\u2010injection of anti\u2010PMN; at this time, anti\u2010PMN\u2010treated rats also demonstrated an improved apomorphine\u2010induced rotational performance. We conclude that anti\u2010PMN treatment could serve as a novel strategy to prevent leakiness to BBB, reduce gliosis, and protect striatal neurons in excitotoxin\u2010injected brain. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/glia.20479","type":"journal-article","created":{"date-parts":[[2007,1,4]],"date-time":"2007-01-04T04:50:07Z","timestamp":1167886207000},"page":"439-451","source":"Crossref","is-referenced-by-count":19,"title":["Depletion of neutrophils reduces neuronal degeneration and inflammatory responses induced by quinolinic acid <i>in vivo<\/i>"],"prefix":"10.1002","volume":"55","author":[{"given":"Jae K.","family":"Ryu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen C.","family":"Tran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James 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