{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:38:19Z","timestamp":1792661899664,"version":"4.5.0"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2008,11,4]],"date-time":"2008-11-04T00:00:00Z","timestamp":1225756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[2009,5]]},"abstract":"Abstract<\/jats:title>\n \n Although there is significant information concerning the consequences of cerebral ischemia on neuronal function, relatively little is known about functional responses of astrocytes, the predominant glial\u2010cell type in the central nervous system. In this study, we asked whether focal ischemia would impact astrocytic Ca\n 2+<\/jats:sup>\n signaling, a characteristic form of excitability in this cell type.\n In vivo<\/jats:italic>\n Ca\n 2+<\/jats:sup>\n imaging of cortical astrocytes was performed using two\u2010photon (2\u2010P) microscopy during the acute phase of photothrombosis\u2010induced ischemia initiated by green light illumination of circulating Rose Bengal. Although whisker evoked potentials were reduced by over 90% within minutes of photothrombosis, astrocytes in the ischemic core remained structurally intact for a few hours.\n In vivo<\/jats:italic>\n Ca\n 2+<\/jats:sup>\n imaging showed that an increase in transient Ca\n 2+<\/jats:sup>\n signals in astrocytes within 20 min of ischemia. These Ca\n 2+<\/jats:sup>\n signals were synchronized and propagated as waves amongst the glial network. Pharmacological manipulations demonstrated that these Ca\n 2+<\/jats:sup>\n signals were dependent on activation of metabotropic glutamate receptor 5 (mGluR5) and metabotropic \u03b3\u2010aminobutyric acid receptor (GABA\n B<\/jats:sub>\n R) but not by P2 purinergic receptor or A1 adenosine receptor. Selective inhibition of Ca\n 2+<\/jats:sup>\n in astrocytes with BAPTA significantly reduced the infarct volume, demonstrating that the enhanced astrocytic Ca\n 2+<\/jats:sup>\n signal contributes to neuronal damage presumably through Ca\n 2+<\/jats:sup>\n \u2010dependent release of glial glutamate. Because astrocytes offer multiple functions in close communication with neurons and vasculature, the ischemia\u2010induced increase in astrocytic Ca\n 2+<\/jats:sup>\n signaling may represent an initial attempt for these cells to communicate with neurons or provide feed back regulation to the vasculature. \u00a9 2008 Wiley\u2010Liss, Inc.\n <\/jats:p>","DOI":"10.1002\/glia.20804","type":"journal-article","created":{"date-parts":[[2008,11,4]],"date-time":"2008-11-04T20:40:20Z","timestamp":1225831220000},"page":"767-776","source":"Crossref","is-referenced-by-count":113,"title":["Photothrombosis ischemia stimulates a sustained astrocytic Ca\n 2+<\/sup>\n signaling\n in vivo<\/i>"],"prefix":"10.1002","volume":"57","author":[{"given":"Shinghua","family":"Ding","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiannan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenju","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip G.","family":"Haydon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,11,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-21-09430.2002"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0473-04.2004"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-17-06822.1998"},{"key":"e_1_2_6_5_1","first-page":"151","article-title":"Biochemistry ischemic stroke","volume":"92","author":"Barber PA","year":"2003","journal-title":"Adv Neurol"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02421.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-01-00183.2002"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2001-07.2007"},{"key":"e_1_2_6_9_1","first-page":"520","volume-title":"Neuroglia","author":"Dirnag U","year":"2004"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.24.1.31"},{"key":"e_1_2_6_11_1","first-page":"1","article-title":"Fine mapping of the spatial relationship between acute ischemia and dendritic structure indicates selective vulnerability of layer V neuron dendritic tufts within single neurons in vivo","volume":"11","author":"Enright LE","year":"2007","journal-title":"J Cerebral Blood Flow 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