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Using confocal morphometric analysis of biocytin\u2010filled astrocytic syncytia in rat hippocampal CA1 <jats:italic>stratum radiatum<\/jats:italic> we found that each astrocyte directly couples, on average, to 11 other astrocytes with a mean interastrocytic distance of 45 \u03bcm. Voltage\u2010independent and bidirectional transjunctional currents were always measured between directly coupled astrocyte pairs in dual voltage\u2010clamp recordings, but never from astrocyte\u2013NG2 glia or astrocyte\u2013interneuron pairs. The electrical coupling ratio varied considerably among astrocytes in developing postnatal day 14 rats (P14, 0.5\u201312.4%, mean = 3.6%), but became more constant in young adult P21 rats (0.18\u20133.9%, mean = 1.6%), and the coupling ratio declined exponentially with increasing pair distance. Electrical coupling was not affected by short\u2010term oxygen\u2013glucose deprivation (OGD) treatment, but showed delayed inhibition in an acidic extracellular pH of 6.4. Combination of acidic pH (6.4) and OGD, a condition that better represents cerebral ischemia <jats:italic>in vivo<\/jats:italic>, accelerated the inhibition of electrical coupling. Our results show that, under physiological conditions, 20.7\u201324.2% of K<jats:sup>+<\/jats:sup> induced currents can travel from any astrocytic soma in CA1 <jats:italic>stratum radiatum<\/jats:italic> to the gap junctions of the nearest neighbor astrocytes, but this should be severely inhibited as a consequence of the OGD and acidosis seen in the ischemic brain. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/glia.20939","type":"journal-article","created":{"date-parts":[[2009,9,30]],"date-time":"2009-09-30T19:47:15Z","timestamp":1254340035000},"page":"481-493","source":"Crossref","is-referenced-by-count":53,"title":["Electrical coupling of astrocytes in rat hippocampal slices under physiological and simulated ischemic 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