{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:55:39Z","timestamp":1791309339825,"version":"4.3.1"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,12,8]],"date-time":"2009-12-08T00:00:00Z","timestamp":1260230400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Production of nerve growth factor (NGF) from Schwann cells (SCs) progressively declines in the distal stump, if axonal regeneration is staggered across the suture site after peripheral nerve injuries. This may be an important factor limiting the outcome of nerve injury repair. Thus far, extensive efforts are devoted to modulating NGF production in cultured SCs, but little has been achieved. In the present\n                    <jats:italic>in vitro<\/jats:italic>\n                    study, electrical stimulation (ES) was attempted to stimulate cultured SCs to release NGF. Our data showed that ES was capable of enhancing NGF release from cultured SCs. An electrical field (1 Hz, 5 V\/cm) caused a 4.1\u2010fold increase in NGF release from cultured SCs. The ES\u2010induced NGF release is calcium dependent. Depletion of extracellular or\/and intracellular calcium partially\/ completely abolished the ES\u2010induced NGF release. Further pharmacological interventions showed that ES induces calcium influx through T\u2010type voltage\u2010gated calcium channels and mobilizes calcium from 1, 4, 5\u2010trisphosphate\u2010sensitive stores and caffeine\/ryanodine\u2010sensitive stores, both of which contributed to the enhanced NGF release induced by ES. In addition, a calcium\u2010triggered exocytosis mechanism was involved in the ES\u2010induced NGF release from cultured SCs. These findings show the feasibility of using ES in stimulating SCs to release NGF, which holds great potential in promoting nerve regeneration by enhancing survival and outgrowth of damaged nerves, and is of great significance in nerve injury repair and neuronal tissue engineering. \u00a9 2009 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/glia.20951","type":"journal-article","created":{"date-parts":[[2009,12,8]],"date-time":"2009-12-08T17:06:25Z","timestamp":1260291985000},"page":"622-631","source":"Crossref","is-referenced-by-count":134,"title":["Electrical stimulation induces calcium\u2010dependent release of NGF from cultured Schwann cells"],"prefix":"10.1002","volume":"58","author":[{"given":"Jinghui","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengxu","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueyu","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuojing","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,12,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2000.01341.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-07-02602.2000"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:CEMN.0000022770.66463.f7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1991.sp018737"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-19-07417.2000"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-23-10399.2002"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1385\/MN:27:3:277"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.F.01437"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-15-06631.2002"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2005.02.007"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","first-page":"a1","DOI":"10.2741\/A733","article-title":"Control 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