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We have recently shown that stimulation of M<jats:sub>3<\/jats:sub>muscarinic receptors in astrocytes increases the synthesis and release of fibronectin, laminin, and plasminogen activator inhibitor\u20101, causing neurite outgrowth in hippocampal neurons. As M<jats:sub>3<\/jats:sub>muscarinic receptor signaling in astroglial cells is strongly inhibited by ethanol, we hypothesized that ethanol may also inhibit neuritogenesis in hippocampal neurons induced by carbachol\u2010stimulated astrocytes. In the present study, we report that the effect of carbachol\u2010stimulated astrocytes on hippocampal neuron neurite outgrowth was inhibited in a concentration\u2010dependent manner (25\u2013100 mM) by ethanol. This effect was because of the inhibition of the release of fibronectin, laminin, and plasminogen activator inhibitor\u20101. Similar effects on neuritogenesis and on the release of astrocyte extracellular proteins were observed after the incubation of astrocytes with carbachol in the presence of 1\u2010butanol, another short\u2010chain alcohol, which like ethanol is a competitive substrate for phospholipase D, but not by<jats:italic>tert<\/jats:italic>\u2010butanol, its analog that is not a substrate for this enzyme. This study identifies a potential novel mechanism involved in the developmental effects of ethanol mediated by the interaction of ethanol with cell signaling in astrocytes, leading to an impairment in neuron\u2013astrocyte communication. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/glia.21015","type":"journal-article","created":{"date-parts":[[2010,5,23]],"date-time":"2010-05-23T17:38:56Z","timestamp":1274636336000},"page":"1395-1406","source":"Crossref","is-referenced-by-count":44,"title":["Ethanol inhibits neuritogenesis induced by astrocyte muscarinic receptors"],"prefix":"10.1002","volume":"58","author":[{"given":"Marina","family":"Guizzetti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadia 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