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We find Claudin k in subcellular compartments consistent with location in autotypic tight junctions of oligodendrocytes and myelinating Schwann cells. Expression starts in the hindbrain at 2 days (mRNA) and 3 days (protein) postfertilization and is maintained in adults. A newly generated<jats:italic>claudin k<\/jats:italic>:green fluorescent protein (GFP) reporter line allowed us to characterize oligodendrocytes in the adult retina that express Claudin k and<jats:italic>olig2<\/jats:italic>, but not P0 and uniquely only form loose wraps of membrane around axons. After a crush of the adult optic nerve, Claudin k protein levels were first reduced and then recovered within 4 weeks postlesion, concomitant with optic nerve myelin de\u2010 and regeneration. During optic nerve regeneration, oligodendrocytes, many of which were newly generated, repopulated the lesion site and exhibited increasing morphological complexity over time. Thus, Claudin k is a novel myelin\u2010associated protein expressed by oligodendrocytes and Schwann cells from early stages of wrapping and myelin formation in zebrafish development and adult regeneration, suggesting important functions of the gene for myelin formation and maintenance. Our Claudin k antibodies and<jats:italic>claudin k<\/jats:italic>:GFP reporter line represent excellent ways to visualize oligodendrocyte and Schwann cell differentiation<jats:italic>in vivo<\/jats:italic>. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/glia.21260","type":"journal-article","created":{"date-parts":[[2011,10,21]],"date-time":"2011-10-21T18:59:54Z","timestamp":1319223594000},"page":"253-270","source":"Crossref","is-referenced-by-count":81,"title":["Claudin k is specifically expressed in cells that form myelin during development of the nervous system and regeneration of the optic nerve in adult zebrafish"],"prefix":"10.1002","volume":"60","author":[{"given":"Eva 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