{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T21:05:07Z","timestamp":1791061507971,"version":"4.1.0"},"reference-count":69,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,1,28]],"date-time":"2013-01-28T00:00:00Z","timestamp":1359331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microglial cells constitute the first line of defense of the central nervous system (CNS) against microbial invasion. Pathogens are detected thanks to an array of innate immune receptors termed pattern recognition receptors (PRRs). PRRs have been thoroughly characterized in bone marrow\u2010derived macrophages, but the PRRs repertoire and functionality in microglial cells remain largely unknown. Microglial cells express various Toll\u2010like Receptors and the Nod1\/2 receptors. Recently, a novel innate immune signalling pathway, the inflammasome pathway has been uncovered. Inflammasome activation leads to caspase\u20101 activation, release of the proinflammatory cytokines, IL\u20101\u03b2 and IL\u201018 and cell death in a process termed pyroptosis. One inflammasome receptor, NLRP3, has been characterized in microglial cells and associated with response to infections and in the initiation of neuro\u2010degeneration in an Alzheimer's disease model. <jats:italic>Legionella pneumophila<\/jats:italic> (<jats:italic>L.pneumophila<\/jats:italic>) is a flagellated bacterium replicating within macrophages. In bone marrow\u2010derived macrophages, <jats:italic>L. pneumophila<\/jats:italic> is detected in a flagellin\u2010dependent manner by the Naip5\u2010NLRC4 (Ipaf) inflammasome pathway. In this study, we decided to use <jats:italic>L. pneumophila<\/jats:italic> to investigate the presence and the functionality of this inflammasome in primary murine microglial cells. We show that microglial cells detect <jats:italic>L. pneumophila<\/jats:italic> infection in a flagellin\u2010dependent manner leading to caspase\u20101\u2010mediated bacterial growth restriction, infected cell death and secretion of the proinflammatory cytokines IL\u20101\u03b2 and IL18. Overall, our data demonstrate that microglial cells have a functional Naip5\u2010NLRC4 inflammasome likely to be important to monitor and clear CNS infections by flagellated bacteria. \u00a9 2013 Wiley Periodicals, Inc. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/glia.22454","type":"journal-article","created":{"date-parts":[[2013,1,28]],"date-time":"2013-01-28T10:31:32Z","timestamp":1359369092000},"page":"539-549","source":"Crossref","is-referenced-by-count":43,"title":["Inflammasome activation restricts <i>Legionella pneumophila<\/i> replication in primary microglial cells through flagellin detection"],"prefix":"10.1002","volume":"61","author":[{"given":"Yvan","family":"Jamilloux","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Pierini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Querenet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carole","family":"Juruj","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010Laure","family":"Fauchais","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Odile","family":"Jauberteau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Jarraud","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9rard","family":"Lina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Etienne","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig R.","family":"Roy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Henry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Davoust","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Ader","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,1,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2010.01.003"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00018"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-008-1063-2"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604933200"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2009.01401.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00012"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2070"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1123511"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbi.2007.10.001"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2011.01.003"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00117-11"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01382-08"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22343"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20770"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2008.01.009"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2008.01.010"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneurol.2010.75"},{"key":"e_1_2_7_19_1","first-page":"69","article-title":"El \u201ctercer elemento\u201d de los centros nerviosos","volume":"9","author":"del Rio Hortega P","year":"1919","journal-title":"Bol Soc Esp Biol"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2005.06.008"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197712012972201"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/brbi.1995.1012"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.06-08-02163.1986"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1636"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2011.07481.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1997"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031220"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/35074106"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1100790"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-198409000-00004"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10558"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00011.2010"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2557-04.2004"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10394"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002371-200203001-00011"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.6A7101.2006"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90490-5"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20968"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0404.2001.00356.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2006.12.001"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-009-9170-6"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1080\/096293502900000131"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051659"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.57.2.342"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.6.3916"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.33.10.1340"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.21104"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.021908.132528"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0020018"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2565"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.4.942-964.2004"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.6.1658-1666.2004"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2008.00496.x"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.040"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2008.06.001"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00905-09"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1159\/000008231"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2006.06.009"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10154"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1179\/096805106X118771"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuint.2006.05.001"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.9.3609-3620.1995"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2004.10.028"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-4453(83)91018-6"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1080\/13550280490523706"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.45.7.603"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-010-9425-2"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2009.08.033"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1305"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10510"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fglia.22454","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.22454","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T16:16:48Z","timestamp":1698682608000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.22454"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1,28]]},"references-count":69,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,4]]}},"alternative-id":["10.1002\/glia.22454"],"URL":"https:\/\/doi.org\/10.1002\/glia.22454","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1,28]]}}}