{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:27:57Z","timestamp":1791160077048,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,10,26]],"date-time":"2013-10-26T00:00:00Z","timestamp":1382745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:p>Oligodendrocytes (OLGs) produce and maintain myelin in the central nervous system (CNS). In the demyelinating autoimmune disease multiple sclerosis, OLGs are damaged and those remaining fail to fully remyelinate CNS lesions. Therefore, current therapies directed to restrain the inflammation process with approaches that protect and reconstitute oligodendrocyte density would be essential to pave the way of myelin repair. A critical signal for oligodendrocytes is insulin\u2010like growth factor\u20101 (IGF\u20101), which promotes their development and ultimately myelin formation. PTEN inhibits the phosphoinositide 3\u2010kinase (PI3K)\/Akt signaling, a convergence downstream pathway for growth factors such as IGF\u20101. In this report, we temporarily inhibited PTEN activity by treating rat and human oligodendrocyte progenitors (OLPs) cultured alone or with dorsal root ganglion neurons (DRGNs) with bisperoxovanadium (phen). Our findings show that phen potentiates IGF\u20101 actions by increasing proliferation of OLPs in a concentration\u2010dependent manner, and caused a sustained and time\u2010dependent activation of the main pathways: PI3K\/Akt\/mammalian target of rapamycin (mTOR) and MEK\/ERK. At low concentrations, IGF\u20101 and phen stimulated the differentiation of rat and human OLPs. Concordantly, the PTEN inhibitor together with IGF\u20101 robustly augmented myelin basic protein accumulation in rat newborn and human fetal OLGs co\u2010cultured with DRGNs in a longer timeframe by promoting the elaboration of organized myelinated fibers as evidenced by confocal microscopy. Thus, our results suggest that a transient suppression of a potential barrier for myelination in combination with other therapeutic approaches including growth factors may be promising to improve the functional recovery of CNS injuries. GLIA 2013;62:64\u201377<\/jats:p>","DOI":"10.1002\/glia.22584","type":"journal-article","created":{"date-parts":[[2013,10,26]],"date-time":"2013-10-26T13:22:42Z","timestamp":1382793762000},"page":"64-77","source":"Crossref","is-referenced-by-count":49,"title":["The PTEN inhibitor bisperoxovanadium enhances myelination by amplifying IGF\u20101 signaling in rat and human oligodendrocyte progenitors"],"prefix":"10.1002","volume":"62","author":[{"given":"Marcio L.","family":"De Paula","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Therapeutics McGill University, Montreal Quebec Canada"},{"name":"Integrated Program in Neuroscience McGill University, Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao\u2010Ling","family":"Cui","sequence":"additional","affiliation":[{"name":"Neuroimmunology Unit, Montreal Neurological Institute McGill University, Montreal Quebec 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