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Astrocytes and microglia are important mediators of A\u03b2 clearance and A\u03b2 aggregation state and the presence of amyloid associated proteins (AAPs), such as Apolipoproteins E and J (ApoE and ApoJ), may influence A\u03b2 clearance by these cells. Here we set out to investigate whether astrocytes and microglia differ in uptake efficiency of A\u03b2 oligomers (A\u03b2<jats:sub>oligo<\/jats:sub>) and A\u03b2 fibrils (A\u03b2<jats:sub>fib<\/jats:sub>), and whether the A\u03b2 aggregation state and\/or presence of AAPs affect A\u03b2 uptake in these cells <jats:italic>in vitro<\/jats:italic>. Adult human primary microglia and astrocytes, isolated from short delay post\u2010mortem brain tissue, were exposed to either A\u03b2<jats:sub>oligo<\/jats:sub> or A\u03b2<jats:sub>fib<\/jats:sub> alone or combined with a panel of certain AAPs whereafter A\u03b2\u2010positive cells were quantified using flow cytometry. Upon exposure to A\u03b2 combined with ApoE, ApoJ, \u03b11\u2010antichymotrypsin (ACT) and a combination of serum amyloid P and complement C1q (SAP\u2010C1q), a clear reduction in astrocytic but not microglial A\u03b2<jats:sub>oligo<\/jats:sub> uptake, was observed. In contrast, A\u03b2<jats:sub>fib<\/jats:sub> uptake was strongly reduced in the presence of AAPs in microglia, but not in astrocytes. These data provide the first evidence of distinct roles of microglia and astrocytes in A\u03b2 clearance. More importantly we show that A\u03b2 clearance by glial cells is negatively affected by AAPs like ApoE and ApoJ. Thus, targeting the association of A\u03b2 with AAPs, such as ApoE and ApoJ, could serve as a therapeutic strategy to increase A\u03b2 clearance by glial cells. 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