{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:04:41Z","timestamp":1791198281604,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,1,30]],"date-time":"2014-01-30T00:00:00Z","timestamp":1391040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>\n                    Network activity in the brain is associated with a transient increase in extracellular K\n                    <jats:sup>+<\/jats:sup>\n                    concentration. The excess K\n                    <jats:sup>+<\/jats:sup>\n                    is removed from the extracellular space by mechanisms proposed to involve Kir4.1\u2010mediated spatial buffering, the Na\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    \/2Cl\n                    <jats:sup>\u2212<\/jats:sup>\n                    cotransporter 1 (NKCC1), and\/or Na\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    \u2010ATPase activity. Their individual contribution to [K\n                    <jats:sup>+<\/jats:sup>\n                    ]\n                    <jats:sub>o<\/jats:sub>\n                    management has been of extended controversy. This study aimed, by several complementary approaches, to delineate the transport characteristics of Kir4.1, NKCC1, and Na\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    \u2010ATPase and to resolve their involvement in clearance of extracellular K\n                    <jats:sup>+<\/jats:sup>\n                    transients. Primary cultures of rat astrocytes displayed robust NKCC1 activity with [K\n                    <jats:sup>+<\/jats:sup>\n                    ]\n                    <jats:sub>o<\/jats:sub>\n                    increases above basal levels. Increased [K\n                    <jats:sup>+<\/jats:sup>\n                    ]\n                    <jats:sub>o<\/jats:sub>\n                    produced NKCC1\u2010mediated swelling of cultured astrocytes and NKCC1 could thereby potentially act as a mechanism of K\n                    <jats:sup>+<\/jats:sup>\n                    clearance while concomitantly mediate the associated shrinkage of the extracellular space. In rat hippocampal slices, inhibition of NKCC1 failed to affect the rate of K\n                    <jats:sup>+<\/jats:sup>\n                    removal from the extracellular space while Kir4.1 enacted its spatial buffering only during a local [K\n                    <jats:sup>+<\/jats:sup>\n                    ]\n                    <jats:sub>o<\/jats:sub>\n                    increase. In contrast, inhibition of the different isoforms of Na\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    \u2010ATPase reduced post\u2010stimulus clearance of K\n                    <jats:sup>+<\/jats:sup>\n                    transients. The astrocyte\u2010characteristic \u03b12\u03b22 subunit composition of Na\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    \u2010ATPase, when expressed in\n                    <jats:italic>Xenopus<\/jats:italic>\n                    oocytes, displayed a K\n                    <jats:sup>+<\/jats:sup>\n                    affinity and voltage\u2010sensitivity that would render this subunit composition specifically geared for controlling [K\n                    <jats:sup>+<\/jats:sup>\n                    ]\n                    <jats:sub>o<\/jats:sub>\n                    during neuronal activity. In rat hippocampal slices, simultaneous measurements of the extracellular space volume revealed that neither Kir4.1, NKCC1, nor Na\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    \u2010ATPase accounted for the stimulus\u2010induced shrinkage of the extracellular space. Thus, NKCC1 plays no role in activity\u2010induced extracellular K\n                    <jats:sup>+<\/jats:sup>\n                    recovery in native hippocampal tissue while Kir4.1 and Na\n                    <jats:sup>+<\/jats:sup>\n                    \/K\n                    <jats:sup>+<\/jats:sup>\n                    \u2010ATPase serve temporally distinct roles. GLIA 2014;62:608\u2013622\n                  <\/jats:p>","DOI":"10.1002\/glia.22629","type":"journal-article","created":{"date-parts":[[2014,1,30]],"date-time":"2014-01-30T10:14:57Z","timestamp":1391076897000},"page":"608-622","source":"Crossref","is-referenced-by-count":269,"title":["Contributions of the Na\n                    <sup>+<\/sup>\n                    \/K\n                    <sup>+<\/sup>\n                    \u2010ATPase, NKCC1, and Kir4.1 to hippocampal K\n                    <sup>+<\/sup>\n                    clearance and volume responses"],"prefix":"10.1002","volume":"62","author":[{"given":"Brian Roland","family":"Larsen","sequence":"first","affiliation":[{"name":"Department of Cellular and Molecular Medicine Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mette","family":"Assentoft","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Medicine Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria L.","family":"Cotrina","sequence":"additional","affiliation":[{"name":"School of Medicine and Dentistry University of Rochester Medical Center Rochester New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan Z.","family":"Hua","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering University of Buffalo Buffalo New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maiken","family":"Nedergaard","sequence":"additional","affiliation":[{"name":"School of Medicine and Dentistry University of Rochester Medical Center Rochester New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Kaila","sequence":"additional","affiliation":[{"name":"Department of Biosciences University of Helsinki Helsinki Finland"},{"name":"Neuroscience Center, University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juha","family":"Voipio","sequence":"additional","affiliation":[{"name":"Department of Biosciences University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nanna","family":"MacAulay","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Medicine Faculty of Health and Medical Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,1,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1252"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22498"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac048799a"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1987.sp016361"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22299"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semnephrol.2005.03.004"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.5.1824"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(94)90264-X"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2078-10.2010"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/12.5.515"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(98)00078-9"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.3.1976"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.00240.2001"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00236151"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051124"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1139\/y87-164"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22319"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.21205"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncom.2013.00114"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013201"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)03254-1"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)55391-6"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.5.1800"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2785716"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.06.008"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440140303"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-013-0986-3"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11064-012-0731-3"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.11-05-01458.1991"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10023"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.11-02-00381.1991"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.20344"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2009.09.072"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.08.053"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6474173"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1981.sp013981"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4835(05)80166-6"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1966.29.4.788"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1997.272.1.C173"},{"key":"e_1_2_6_41_1","doi-asserted-by":"crossref","first-page":"C1267","DOI":"10.1152\/ajpcell.1997.273.4.C1267","article-title":"Partial 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