{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:55:16Z","timestamp":1785412516324,"version":"3.56.0"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,7,2]],"date-time":"2014-07-02T00:00:00Z","timestamp":1404259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"crossref","award":["FP7-MC-214003-2"],"award-info":[{"award-number":["FP7-MC-214003-2"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"crossref","award":["FP7-278612"],"award-info":[{"award-number":["FP7-278612"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:p>Fibroblast growth factor 2 (FGF\u20102) is a trophic factor expressed by glial cells and different neuronal populations. Addition of FGF\u20102 to spinal cord and dorsal root ganglia (DRG) explants demonstrated that FGF\u20102 specifically increases motor neuron axonal growth. To further explore the potential capability of FGF\u20102 to promote axon regeneration, we produced a lentiviral vector (LV) to overexpress FGF\u20102 (LV\u2010FGF2) in the injured rat peripheral nerve. Cultured Schwann cells transduced with FGF\u20102 and added to collagen matrix embedding spinal cord or DRG explants significantly increased motor but not sensory neurite outgrowth. LV\u2010FGF2 was as effective as direct addition of the trophic factor to promote motor axon growth <jats:italic>in vitro<\/jats:italic>. Direct injection of LV\u2010FGF2 into the rat sciatic nerve resulted in increased expression of FGF\u20102, which was localized in the basal lamina of Schwann cells. To investigate the <jats:italic>in vivo<\/jats:italic> effect of FGF\u20102 overexpression on axonal regeneration after nerve injury, Schwann cells transduced with LV\u2010FGF2 were grafted in a silicone tube used to repair the resected rat sciatic nerve. Electrophysiological tests conducted for up to 2 months after injury revealed accelerated and more marked reinnervation of hindlimb muscles in the animals treated with LV\u2010FGF2, with an increase in the number of motor and sensory neurons that reached the distal tibial nerve at the end of follow\u2010up. GLIA 2014;62:1736\u20131746<\/jats:p>","DOI":"10.1002\/glia.22712","type":"journal-article","created":{"date-parts":[[2014,7,2]],"date-time":"2014-07-02T15:06:52Z","timestamp":1404313612000},"page":"1736-1746","source":"Crossref","is-referenced-by-count":57,"title":["Schwann cells transduced with a lentiviral vector encoding Fgf\u20102 promote motor neuron regeneration following sciatic nerve injury"],"prefix":"10.1002","volume":"62","author":[{"given":"Ilary","family":"Allodi","sequence":"first","affiliation":[{"name":"Institute of Neurosciences and Department of Cell Biology Physiology and Immunology, Universitat Aut\u00f2noma de Barcelona, and Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades Neurodegenerativas (CIBERNED)  Bellaterra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasil","family":"Mecollari","sequence":"additional","affiliation":[{"name":"Department of Neuroregeneration Netherlands Institute for Neurosciences  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Gonz\u00e1lez\u2010P\u00e9rez","sequence":"additional","affiliation":[{"name":"Institute of Neurosciences and Department of Cell Biology Physiology and Immunology, Universitat Aut\u00f2noma de Barcelona, and Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades Neurodegenerativas (CIBERNED)  Bellaterra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruben","family":"Eggers","sequence":"additional","affiliation":[{"name":"Department of Neuroregeneration Netherlands Institute for Neurosciences  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Hoyng","sequence":"additional","affiliation":[{"name":"Department of Neuroregeneration Netherlands Institute for Neurosciences  Amsterdam The Netherlands"},{"name":"Department of Neurosurgery Leiden University Medical Center Albinusdreef 2 Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joost","family":"Verhaagen","sequence":"additional","affiliation":[{"name":"Department of Neuroregeneration Netherlands Institute for Neurosciences  Amsterdam The Netherlands"},{"name":"Center for Neurogenomics and Cognitive Research Vrije Universiteit Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Navarro","sequence":"additional","affiliation":[{"name":"Institute of Neurosciences and Department of Cell Biology Physiology and Immunology, Universitat Aut\u00f2noma de Barcelona, and Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades Neurodegenerativas (CIBERNED)  Bellaterra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Udina","sequence":"additional","affiliation":[{"name":"Institute of Neurosciences and Department of Cell Biology Physiology and Immunology, Universitat Aut\u00f2noma de Barcelona, and Centro de Investigaci\u00f3n Biom\u00e9dica en Red sobre Enfermedades Neurodegenerativas (CIBERNED)  Bellaterra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,2]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1090940210"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-012-8389-z"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2011.03.006"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2012.05.005"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1525-0016(03)00097-2"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2010.10.002"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/neu.1998.15.387"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071076"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2008.05.018"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0069987"},{"key":"e_1_2_6_12_1","first-page":"187","article-title":"Histologic assessment of sciatic nerve regeneration following resection and graft or tube repair in the mouse","volume":"10","author":"Gomez N","year":"1996","journal-title":"Restor Neurol Neurosci"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6156-10.2011"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20141"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2005.06.020"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1620-06.2006"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2009.08.025"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2005.01.016"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34816"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/neu.20265"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2009.06.003"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2010.0507"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20192"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhsu.2000.4165"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652311794940764"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/iju.12078"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5259.263"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7742(09)87006-2"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2001-2-3-reviews3005"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.23490"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2012.04.004"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2013.10.004"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986712803833218"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410380313"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-1136(200012)32:3<234::AID-GLIA40>3.0.CO;2-3"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1227\/01.neu.0000306108.78044.a2"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2008.06452.x"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20025"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2003.11.070"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.20635"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fglia.22712","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.22712","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T06:02:24Z","timestamp":1696485744000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.22712"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,2]]},"references-count":39,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1002\/glia.22712"],"URL":"https:\/\/doi.org\/10.1002\/glia.22712","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,2]]}}}