{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T00:59:56Z","timestamp":1792112396689,"version":"4.4.0"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81030020 and 30770660"],"award-info":[{"award-number":["81030020 and 30770660"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2006 CB943702"],"award-info":[{"award-number":["2006 CB943702"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>\n                    To determine whether reactive astrocytes stimulated by brain injury can transdifferentiate into functional new neurons, we labeled these cells by injecting a glial fibrillary acidic protein (GFAP) targeted enhanced green fluorescence protein plasmid (pGfa2\u2010eGFP plasmid) into the striatum of adult rats immediately following a transient middle cerebral artery occlusion (MCAO) and performed immunolabeling with specific neuronal markers to trace the neural fates of eGFP\u2010expressing (GFP\n                    <jats:sup>+<\/jats:sup>\n                    ) reactive astrocytes. The results showed that a portion of striatal GFP\n                    <jats:sup>+<\/jats:sup>\n                    astrocytes could transdifferentiate into immature neurons at 1 week after MCAO and mature neurons at 2 weeks as determined by double staining GFP\u2010expressing cells with \u03b2III\u2010tubulin (GFP\n                    <jats:sup>+<\/jats:sup>\n                    \u2010Tuj\u20101\n                    <jats:sup>+<\/jats:sup>\n                    ) and microtubule associated protein\u20102 (GFP\n                    <jats:sup>+<\/jats:sup>\n                    \u2010MAP\u20102\n                    <jats:sup>+<\/jats:sup>\n                    ), respectively. GFP\n                    <jats:sup>+<\/jats:sup>\n                    neurons further expressed choline acetyltransferase, glutamic acid decarboxylase, dopamine receptor D2\u2010like family proteins, and the\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methyl\u2010\n                    <jats:sc>d<\/jats:sc>\n                    \u2010aspartate receptor subunit R2, indicating that astrocyte\u2010derived neurons could develop into cholinergic or GABAergic neurons and express dopamine and glutamate receptors on their membranes. Electron microscopy analysis indicated that GFP\n                    <jats:sup>+<\/jats:sup>\n                    neurons could form synapses with other neurons at 13 weeks after MCAO. Electrophysiological recordings revealed that action potentials and active postsynaptic currents could be recorded in the neuron\u2010like GFP\n                    <jats:sup>+<\/jats:sup>\n                    cells but not in the astrocyte\u2010like GFP\n                    <jats:sup>+<\/jats:sup>\n                    cells, demonstrating that new GFP\n                    <jats:sup>+<\/jats:sup>\n                    neurons possessed the capacity to fire action potentials and receive synaptic inputs. These results demonstrated that striatal astrocyte\u2010derived new neurons participate in the rebuilding of functional neural networks, a fundamental basis for brain repair after injury. These results may lead to new therapeutic strategies for enhancing brain repair after ischemic stroke. GLIA 2015;63:1660\u20131670\n                  <\/jats:p>","DOI":"10.1002\/glia.22837","type":"journal-article","created":{"date-parts":[[2015,6,2]],"date-time":"2015-06-02T01:08:22Z","timestamp":1433207302000},"page":"1660-1670","source":"Crossref","is-referenced-by-count":96,"title":["Striatal astrocytes transdifferentiate into functional mature neurons following ischemic brain injury"],"prefix":"10.1002","volume":"63","author":[{"given":"Chun\u2010Ling","family":"Duan","sequence":"first","affiliation":[{"name":"Department of Neurobiology, School of Basic Medical Science Shanghai Medical College, Fudan University Shanghai People's Republic of China"},{"name":"Research Unit of Brain Injury, State Key Laboratory of Medical Neurobiology Shanghai Medical College, Fudan University Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong\u2010Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Basic Medical Science Shanghai Medical College, Fudan University Shanghai People's Republic of China"},{"name":"Research Unit of Brain Injury, State Key Laboratory of Medical Neurobiology Shanghai Medical College, Fudan University Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu\u2010Wen","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Basic Medical Science Shanghai Medical College, Fudan University Shanghai People's Republic of China"},{"name":"Research Unit of Brain Injury, State Key Laboratory of Medical Neurobiology Shanghai Medical College, Fudan University Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhang","family":"Yu","sequence":"additional","affiliation":[{"name":"The Electron Microscopy Core Laboratory, School of Basic Medical Science Shanghai Medical College, Fudan University Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Lin","family":"Mo","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Basic Medical Science Shanghai Medical College, Fudan University Shanghai People's Republic of China"},{"name":"Research Unit of Brain Injury, State Key Laboratory of Medical Neurobiology Shanghai Medical College, Fudan University Shanghai People's Republic of China"},{"name":"Research Institute of Aging Biology Research Center on Aging and Medicine and Institutes for Biomedical Science, Shanghai Medical College, Fudan University Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian\u2010Hua","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Basic Medical Science Shanghai Medical College, Fudan University Shanghai People's Republic of China"},{"name":"Research Unit of Brain Injury, State Key Laboratory of Medical Neurobiology Shanghai Medical College, Fudan University Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng\u2010Yan","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, School of Basic Medical Science Shanghai Medical College, Fudan University Shanghai People's Republic of China"},{"name":"Research Unit of Brain Injury, State Key Laboratory of Medical Neurobiology Shanghai Medical College, Fudan University Shanghai People's Republic of China"},{"name":"Research Institute of Aging Biology Research Center on Aging and Medicine and Institutes for Biomedical Science, Shanghai Medical College, Fudan University Shanghai People's Republic of 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