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A hallmark of A\u2013T pathology is progressive loss of cerebellar neurons, but the mechanisms that impact neuronal survival are unclear. We now provide a possible mechanism by which A\u2013T astroglia affect the survival of cerebellar neurons. As astroglial functions are difficult to study in an <jats:italic>in vivo<\/jats:italic> setting, particularly in the cerebellum where these cells are intertwined with the far more numerous neurons, we conducted <jats:italic>in vitro<\/jats:italic> coculture experiments that allow for the generation and pharmacological manipulation of purified cell populations. Our analyses revealed that cerebellar astroglia isolated from Atm mutant mice show decreased expression of the cystine\/glutamate exchanger subunit xCT, glutathione (GSH) reductase, and glutathione\u2010S\u2010transferase. We also found decreased levels of intercellular and secreted GSH in A\u2013T astroglia. Metabolic labeling of <jats:sc>l<\/jats:sc>\u2010cystine, the major precursor for GSH, revealed that a key component of the defect in A\u2013T astroglia is an impaired ability to import this rate\u2010limiting precursor for the production of GSH. This impairment resulted in suboptimal extracellular GSH supply, which in turn impaired survival of cerebellar neurons. We show that by circumventing the xCT\u2010dependent import of <jats:sc>l<\/jats:sc>\u2010cystine through addition of <jats:italic>N<\/jats:italic>\u2010acetyl\u2010<jats:sc>l<\/jats:sc>\u2010cysteine (NAC) as an alternative cysteine source, we were able to restore GSH levels in A\u2013T mutant astroglia providing a possible future avenue for targeted therapeutic intervention. 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