{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:06:15Z","timestamp":1790647575731,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,10,11]],"date-time":"2016-10-11T00:00:00Z","timestamp":1476144000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Glia"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:p>Ror2 receptor tyrosine kinase plays crucial roles in developmental morphogenesis and tissue\u2010\/organo\u2010genesis. In the developing brain, Ror2 is expressed in neural stem\/progenitor cells (NPCs) and involved in the regulation of their stemness. However, it remains largely unknown about its role in the adult brain. In this study, we show that <jats:italic>Ror2<\/jats:italic> is up\u2010regulated in reactive astrocytes in the neocortices within 3 days following stab\u2010wound injury. Intriguingly, Ror2\u2010expressing astrocytes were detected primarily at the area surrounding the injury site, where astrocytes express Nestin, a marker of NPCs, and proliferate in response to injury. Furthermore, we show by using astrocyte\u2010specific <jats:italic>Ror2<\/jats:italic> knockout (KO) mice that a loss of <jats:italic>Ror2<\/jats:italic> in astrocytes attenuates injury\u2010induced proliferation of reactive astrocytes. It was also found that basic fibroblast growth factor (bFGF) is strongly up\u2010regulated at 1 day post injury in the neocortices, and that stimulation of cultured quiescent astrocytes with bFGF restarts their cell cycle and induces expression of <jats:italic>Ror2<\/jats:italic> during the G1 phase predominantly in proliferating cells. By using this culture method, we further show that the proportions of <jats:italic>Ror2<\/jats:italic>\u2010expressing astrocytes increase following treatment with the histone deacetylases inhibitors including valproic acid, and that bFGF stimulation increases the levels of <jats:italic>Ror2<\/jats:italic> expression within the respective cells. Moreover, we show that bFGF\u2010induced cell cycle progression into S phase is inhibited or promoted in astrocytes from <jats:italic>Ror2<\/jats:italic> KO mice or NPCs stably expressing <jats:italic>Ror2\u2010GFP<\/jats:italic>, respectively. Collectively, these findings indicate that Ror2 plays a critical role in regulating the cell cycle progression of reactive astrocytes following brain injury, GLIA 2016. 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