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Its permanent alteration by status epilepticus (SE) is associated with learning and cognitive impairments. The mechanisms underlying the initiation of altered neurogenesis after SE are not understood. Glial fibrillary acidic protein\u2010positive radial glia (RG)\u2010like cells proliferate early after SE, but their proliferation dynamics and signaling are largely unclear. We have previously reported a polarized distribution of AMPA receptors (AMPARs) on RG\u2010like cells in vivo and postulated that these may signal their proliferation. Here, we examined the acute effects of kainate on hippocampal precursor cells in vitro and in kainate\u2010induced SE on proliferating and quiescent clones of 5\u2010bromo\u20102\u2010deoxyuridine prelabeled hippocampal precursors in vivo. In vitro, we found that 5 \u03bcM kainate shortened the cell cycle time of RG\u2010like cells via AMPAR activation and accelerated cell cycle re\u2010entry of their progeny. It also shifted their fate choice expanding the population of RG\u2010like cells and reducing the population of downstream amplifying neural progenitors. Kainate enhanced the survival of all precursor cell subtypes. Pharmacologically, kainate's proliferative and survival effects were abolished by AMPAR blockade. Functional AMPAR expression was confirmed on RG\u2010like cells in vitro. In agreement with these observations<jats:italic>,<\/jats:italic> kainate\/seizures enhanced the proliferation and expansion predominantly of constitutively cycling RG\u2010like cell clones in vivo. Our results identify AMPARs as key potential players in initiating the proliferation of dentate RG\u2010like cells and unravel a possible receptor target for modifying the radial glia\u2010like cell response to SE.<\/jats:p>","DOI":"10.1002\/glia.23479","type":"journal-article","created":{"date-parts":[[2018,10,25]],"date-time":"2018-10-25T08:36:13Z","timestamp":1540456573000},"page":"2397-2413","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["AMPA receptors and seizures mediate hippocampal radial glia\u2010like stem cell proliferation"],"prefix":"10.1002","volume":"66","author":[{"given":"Anan","family":"Shtaya","sequence":"first","affiliation":[{"name":"Neurosciences Research Centre, Molecular and Clinical Sciences Research Institute, St George's University of London  London United Kingdom"},{"name":"University of Southampton School of Medicine  Southampton United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed\u2010Ramadan","family":"Sadek","sequence":"additional","affiliation":[{"name":"University of Southampton School of Medicine  Southampton United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malik","family":"Zaben","sequence":"additional","affiliation":[{"name":"University of Southampton School of Medicine  Southampton United Kingdom"},{"name":"Institute of Psychological Medicine and Clinical Neurosciences Cardiff University  Cardiff United Kingdom"},{"name":"Neuroscience and Mental Health Research Institute Cardiff University  Cardiff United Kingdom"},{"name":"B.R.A.I.N. 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