{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T15:21:20Z","timestamp":1783092080723,"version":"3.54.6"},"reference-count":45,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,11,30]],"date-time":"2018-11-30T00:00:00Z","timestamp":1543536000000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Glia"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neuroinflammatory diseases such as multiple sclerosis are characterized by infiltration of lymphocytes into the central nervous system followed by demyelination and axonal degeneration. While evidence suggests that activated T lymphocytes induce neurotoxicity and impair function of neural stem cells, the effect of T cells on oligodendrocyte progenitor cells (OPCs) is still uncertain, partly due to the difficulty in obtaining human OPCs. Here we studied the effect of activated T cells on OPCs using OPCs derived from human hematopoietic stem cells or from human fetal brain. OPCs were exposed to supernatants (sups) from activated T cells. Cell proliferation was determined by EdU incorporation and CellQuanti\u2010Blue assays. Surprisingly, we found that sups from activated T cells induced OPC proliferation by regulating cell cycle progression. Vascular endothelial growth factor A (VEGF\u2010A) transcripts were increased in T cells after activation. Immunodepletion of VEGF\u2010A from activated T cell sups significantly attenuated its effect on OPC proliferation. Furthermore, VEGF receptor 2 (VEGFR2) was expressed on OPCs and its inhibition also attenuated activated T cell\u2010induced OPC proliferation. Thus, activated T cells have a trophic role by promoting OPC proliferation via the VEGFR2 pathway.<\/jats:p>","DOI":"10.1002\/glia.23501","type":"journal-article","created":{"date-parts":[[2018,11,30]],"date-time":"2018-11-30T15:13:18Z","timestamp":1543590798000},"page":"2503-2513","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Activated T cells induce proliferation of oligodendrocyte progenitor cells via release of vascular endothelial cell growth factor\u2010A"],"prefix":"10.1002","volume":"66","author":[{"given":"Elliot H.","family":"Choi","sequence":"first","affiliation":[{"name":"Section of Infections of the Nervous System National Institute of Neurological Disorders and Stroke, National Institutes of Health  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yadi","family":"Xu","sequence":"additional","affiliation":[{"name":"Section of Infections of the Nervous System National Institute of Neurological Disorders and Stroke, National Institutes of Health  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Medynets","sequence":"additional","affiliation":[{"name":"Translational Neuroscience Center National Institute of Neurological Disorders and Stroke, National Institutes of Health  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Chiara G.","family":"Monaco","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Medicine and Neuroscience National Institute of Neurological Disorders and Stroke, National Institutes of Health  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugene O.","family":"Major","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Medicine and Neuroscience National Institute of Neurological Disorders and Stroke, National Institutes of Health  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avindra","family":"Nath","sequence":"additional","affiliation":[{"name":"Section of Infections of the Nervous System National Institute of Neurological Disorders and Stroke, National Institutes of Health  Bethesda Maryland"},{"name":"Translational Neuroscience Center National Institute of Neurological Disorders and Stroke, National Institutes of Health  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0195-5661","authenticated-orcid":false,"given":"Tongguang","family":"Wang","sequence":"additional","affiliation":[{"name":"Translational Neuroscience Center National Institute of Neurological Disorders and Stroke, National Institutes of Health  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2012-0356OC"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI60842"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20848"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20011"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa010994"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.10038"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.092284399"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn917"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2480"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22838"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/pmj.79.927.11"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80359-1"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.02.021"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1944-11.2011"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/60.10.1004"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1308673110"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1998.02563.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-444-52001-2.00002-9"},{"key":"e_1_2_7_20_1","first-page":"1929","article-title":"Subfoveal fibrovascular membranes in age\u2010related macular degeneration express vascular endothelial growth factor","volume":"37","author":"Kvanta A.","year":"1996","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1646"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1999.7224"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2010.12.001"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-011-1018-y"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.072348"},{"key":"e_1_2_7_26_1","first-page":"e4274","article-title":"Progenitor\u2010derived oligodendrocyte culture system from human fetal brain","volume":"70","author":"Monaco M. C.","year":"2012","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.7.4618"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.80.3.241"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/2051-5960-1-85"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410330203"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199906000-00006"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/61.10.914"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.05.018"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.21222"},{"key":"e_1_2_7_35_1","first-page":"782","article-title":"Oligodendrocyte precursors induce early blood\u2010brain barrier opening after white matter injury","volume":"123","author":"Seo J. H.","year":"2013","journal-title":"The Journal of Clinical Investigation"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902773"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.03.003"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000227922.22293.93"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1408295111"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-5022fje"},{"key":"e_1_2_7_41_1","first-page":"8","article-title":"Derivation of neural stem cells from human adult peripheral CD34+ cells for an autologous model of neuroinflammation","volume":"81720","author":"Wang T.","year":"2013","journal-title":"PLoS One"},{"key":"e_1_2_7_42_1","first-page":"e52298","article-title":"Direct induction of human neural stem cells from peripheral blood hematopoietic progenitor cells","volume":"95","author":"Wang T.","year":"2015","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.18-02-00601.1998"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awf031"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.04.002"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2010.06.014"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fglia.23501","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.23501","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/glia.23501","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.23501","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T22:27:13Z","timestamp":1694212033000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.23501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11]]},"references-count":45,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1002\/glia.23501"],"URL":"https:\/\/doi.org\/10.1002\/glia.23501","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11]]},"assertion":[{"value":"2017-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}