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Therefore, OPCs are also termed NG2 glia. Their fate during development has been investigated systematically in several genetically modified mouse models. Consensus exists that postnatal NG2 glia are restricted to the oligodendrocyte (OL) lineage, while, at least in the forebrain, embryonic NG2 glia could also generate astrocytes. In addition, experimental evidence for a neurogenic potential of NG2 glia in the early embryonic brain (before E16.5) has been provided. However, this observation is still controversial. Here, we took advantage of reliable transgene expression in NG2\u2010EYFP and NG2\u2010CreERT2 knock\u2010in mice to study the fate of early embryonic NG2 glia. While pericytes were the main cells with robust NG2 gene activity at E12.5, only a few OPCs expressed NG2 at this early stage of embryogenesis. Subsequently, this proportion of OPCs increased from 3% (E12.5) to 11% and 25% at E14.5 and E17.5, respectively. When Cre DNA recombinase activity was induced at E12.5 and E14.5 and pups were analyzed at postnatal day 0 (P0) and P10, the vast majority of recombined cells, besides pericytes, belonged to the OL lineage cells, with few astrocytes in the ventral forebrain. In other brain regions such as brain stem, cerebellum, and olfactory bulb only OL lineage cells were detected. Therefore, we conclude that NG2 glia from early embryonic brain are restricted to a gliogenic fate and do not differentiate into neurons after birth.<\/jats:p>","DOI":"10.1002\/glia.23590","type":"journal-article","created":{"date-parts":[[2019,2,6]],"date-time":"2019-02-06T12:54:22Z","timestamp":1549457662000},"page":"1094-1103","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Early embryonic NG2 glia are exclusively gliogenic and do not generate neurons in the brain"],"prefix":"10.1002","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9865-0375","authenticated-orcid":false,"given":"Wenhui","family":"Huang","sequence":"first","affiliation":[{"name":"Molecular Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM) University of Saarland  Homburg 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University of Saarland  Homburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2324-2761","authenticated-orcid":false,"given":"Frank","family":"Kirchhoff","sequence":"additional","affiliation":[{"name":"Molecular Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM) University of Saarland  Homburg 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