{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T12:33:40Z","timestamp":1768566820614,"version":"3.49.0"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T00:00:00Z","timestamp":1577059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSCF 31970921"],"award-info":[{"award-number":["NSCF 31970921"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81901378"],"award-info":[{"award-number":["81901378"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31921003"],"award-info":[{"award-number":["31921003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005230","name":"Natural Science Foundation of Chongqing","doi-asserted-by":"publisher","award":["cstc2018jcyjAX0702"],"award-info":[{"award-number":["cstc2018jcyjAX0702"]}],"id":[{"id":"10.13039\/501100005230","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["201503170411"],"award-info":[{"award-number":["201503170411"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013327","name":"Intercollegiate Center for Management Science","doi-asserted-by":"publisher","award":["P09\u2010022\u2010F"],"award-info":[{"award-number":["P09\u2010022\u2010F"]}],"id":[{"id":"10.13039\/100013327","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Glia"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>As the most abundant gap junction protein in the central nervous system (CNS), astrocytic connexin 43 (Cx43) maintains astrocyte network homeostasis, affects oligodendroglial development and participates in CNS pathologies as well as injury progression. However, its role in remyelination is not yet fully understood. To address this issue, we used astrocyte\u2010specific Cx43 conditional knockout (Cx43 cKO) mice generated through the use of a hGFAP\u2010cre promoter, in combination with mice carrying a floxed Cx43 allele that were subjected to lysolecithin so as to induce demyelination. We found no significant difference in the demyelination of the corpus callosum between Cx43 cKO mice and their non\u2010cre littermate controls, while the remyelination process in Cx43 cKO mice was accelerated. Moreover, an increased number of mature oligodendrocytes and an unaltered number of oligodendroglial lineage cells were found in Cx43 cKO mouse lesions. This indicates that oligodendrocyte precursor cell (OPC) differentiation was facilitated by astroglial Cx43 depletion as remyelination progressed. Underlying the latter, there was a down\u2010regulated glial activation and modulated local inflammation as well as a reduction of myelin debris in Cx43 cKO mice. Importantly, 2\u2009weeks of orally administrating boldine, a natural alkaloid that blocks Cx hemichannel activity in astrocytes without affecting gap junctional communication, obviously modulated local inflammation and promoted remyelination. Together, the data suggest that the astrocytic Cx43 hemichannel is negatively involved in the remyelination process by favoring local inflammation. Consequently, inhibiting Cx43 hemichannel functionality may be a potential therapeutic approach for demyelinating diseases in the CNS.<\/jats:p>","DOI":"10.1002\/glia.23770","type":"journal-article","created":{"date-parts":[[2019,12,23]],"date-time":"2019-12-23T12:36:24Z","timestamp":1577104584000},"page":"1201-1212","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Connexin 43 deletion in astrocytes promotes CNS remyelination by modulating local inflammation"],"prefix":"10.1002","volume":"68","author":[{"given":"Tao","family":"Li","sequence":"first","affiliation":[{"name":"Department of Histology and Embryology, Institute of Brain and Intelligence Army Medical University (Third Military Medical University)  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianqin","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Institute of Brain and Intelligence Army Medical University (Third Military Medical University)  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangdan","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Institute of Brain and Intelligence Army Medical University (Third Military Medical University)  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pascal","family":"Ezan","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Research in Biology (CIRB) Coll\u00e8ge de France  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenju","family":"Yi","sequence":"additional","affiliation":[{"name":"Seventh Affiliated Hospital of Sun Yat\u2010sen University  Shenzhen China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaorui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Institute of Brain and Intelligence Army Medical University (Third Military Medical University)  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette","family":"Koulakoff","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Research in Biology (CIRB) Coll\u00e8ge de France  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Institute of Brain and Intelligence Army Medical University (Third Military Medical University)  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianjun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Institute of Brain and Intelligence Army Medical University (Third Military Medical University)  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan C.","family":"S\u00e1ez","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda Pontificia Universidad Cat\u00f3lica de Chile  Santiago Chile"},{"name":"Instituto de Neurociencias Centro Interdisciplinario de Neurociencias de Valpara\u00edso  Valpara\u00edso Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Giaume","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Research in Biology (CIRB) Coll\u00e8ge de France  Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0391-6909","authenticated-orcid":false,"given":"Lan","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Institute of Brain and Intelligence Army Medical University (Third Military Medical University)  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,12,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22785"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-008-9053-y"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23637"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23693"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa010994"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2010.07.014"},{"key":"e_1_2_9_8_1","first-page":"71","article-title":"Oligodendrocyte, astrocyte, and microglia crosstalk in myelin development, damage, and repair","volume":"4","author":"Domingues H. S.","year":"2016","journal-title":"Frontiers in Cell and Development Biology"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2480"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-008-1004-6"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004647-200204000-00009"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2013.00088"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.05-5234fje"},{"issue":"1","key":"e_1_2_9_14_1","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1242\/jcs.13.1.257","article-title":"A quantitative analysis of the effects of the intraneural injection of lysophosphatidyl choline","volume":"13","author":"Gregson N. A.","year":"1973","journal-title":"Journal of Cell Science"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.03.007"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/593672"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2990.2003.00491.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1216-11.2012"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apcsb.2015.12.001"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2016.03.009"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2615-05.2006"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awn096"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20141656"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23450"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-18-07046.2001"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3618"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1493-16.2016"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1159\/000112150"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3469"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.12705"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.13195"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2015.06.010"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100913-013101"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.178731"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2009.12.040"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2010.03.004"},{"key":"e_1_2_9_37_1","doi-asserted-by":"crossref","unstructured":"Ransom B. R. G. C.(2013).Gap Junctions Hemichannels.H. R.Kettenmann&B. R.Ransom(Eds.) Neuroglia(5th ed. Vol.24 pp.292\u2013305).Oxford UK:Oxford University Press.","DOI":"10.1093\/med\/9780199794591.003.0024"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/948976"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2042-07.2007"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2006.00035.x"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22627"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-014-9070-5"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00042.2016"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-018-1061-y"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23182"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2016.63"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fglia.23770","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.23770","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/glia.23770","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.23770","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T07:00:46Z","timestamp":1693897246000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.23770"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,23]]},"references-count":45,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["10.1002\/glia.23770"],"URL":"https:\/\/doi.org\/10.1002\/glia.23770","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,23]]},"assertion":[{"value":"2019-03-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}