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Nevertheless, various age\u2010related pathologies such as neurocognitive disorders have emerged as serious complications. A \u201cshock and kill\u201d strategy using latency\u2010reversing agents (LRA) to reactivate HIV\u20101 has been proposed to eliminate the viral reservoir in such chronically infected patients. However, the impact of LRA on the central nervous system remains elusive. Given that an increased amyloid beta (A\u03b2) deposition is a feature of HIV\u20101\u2010infected brains, we investigated the consequences of HIV\u20101 infection and treatment with two LRA (bryostatin\u20101 and JQ1) on the capacity of human astrocytes to engulf and clear A\u03b2. We show here that HIV\u20101\u2010infected astrocytes accumulate a very high amount of A\u03b2 compared to uninfected cells, but the engulfed peptide in degraded very slowly. The LRA bryostatin\u20101 induces a reduction in A\u03b2 endocytosis, whereas JQ1 treatment results in a very slow degradation of the ingested material associated with a reduced expression of the endopeptidase neprilysin. An exposure to JQ1 also induces a sustained release of A\u03b2\u2010loaded microvesicles. Thus, both HIV\u20101 infection and treatment with some LRA could contribute to the reported A\u03b2 accumulation in the brain of HIV\u20101\u2010infected persons.<\/jats:p>","DOI":"10.1002\/glia.23833","type":"journal-article","created":{"date-parts":[[2020,4,6]],"date-time":"2020-04-06T14:12:06Z","timestamp":1586182326000},"page":"2212-2227","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["<scp>HIV<\/scp>\u20101 infection and latency\u2010reversing agents bryostatin\u20101 and <scp>JQ1<\/scp> disrupt amyloid beta homeostasis in human astrocytes"],"prefix":"10.1002","volume":"68","author":[{"given":"Aliz\u00e9","family":"Proust","sequence":"first","affiliation":[{"name":"Axe des Maladies Infectieuses et Immunitaires, Centre de Recherche du CHU de Qu\u00e9bec\u2010Universit\u00e9 Laval, Pavillon CHUL  Qu\u00e9bec QC 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