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Furthermore, clinical studies have shown that antagonists of CB1 can increase the incidence and severity of major depressive episodes. However, the underlying pathomechanisms are largely unknown. In this study, we have focused on the possible involvement of astrocytes. Using the highly sensitive RNAscope technology, we show for the first time that a subpopulation of astrocytes in the adult mouse brain expresses <jats:italic>Dagla<\/jats:italic>, albeit at low levels. Targeted lipidomics revealed that astrocytic DAGLa only accounts for a minor percentage of the steady\u2010state brain 2\u2010AG levels and other arachidonic acid derived lipids like prostaglandins. Nevertheless, the deletion of <jats:italic>Dagla<\/jats:italic> in adult mouse astrocytes had profound behavioral consequences with significantly increased depressive\u2010like behavioral responses and striking effects on maternal behavior, corresponding with increased levels of serum progesterone and estradiol. Our findings therefore indicate that lipids from the DAGLa metabolic axis in astrocytes play a key regulatory role in affective behaviors.<\/jats:p>","DOI":"10.1002\/glia.23903","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T15:59:39Z","timestamp":1599062379000},"page":"377-391","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Diacylglycerol lipase alpha in astrocytes is involved in maternal care and affective behaviors"],"prefix":"10.1002","volume":"69","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1619-7584","authenticated-orcid":false,"given":"Lena\u2010Louise","family":"Sch\u00fcle","sequence":"first","affiliation":[{"name":"Institute of Molecular Psychiatry, Medical Faculty University of Bonn  Bonn Germany"},{"name":"Rheinische Friedrich\u2010Wilhelms\u2010Universit\u00e4t Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Glasmacher","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Medicine University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrg","family":"Gertsch","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Medicine University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie Denise","family":"Roggan","sequence":"additional","affiliation":[{"name":"Institute of Molecular Psychiatry, Medical Faculty University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janis\u2010Lisa","family":"Transfeld","sequence":"additional","affiliation":[{"name":"Institute of Molecular Psychiatry, Medical Faculty University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Bindila","sequence":"additional","affiliation":[{"name":"Institute of Physiological Chemistry University Medical Center of the Johannes Gutenberg University of Mainz  Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beat","family":"Lutz","sequence":"additional","affiliation":[{"name":"Institute of Physiological Chemistry University Medical Center of the Johannes Gutenberg University of Mainz  Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl\u2010Christian","family":"Kolbe","sequence":"additional","affiliation":[{"name":"Institute of Innate Immunity University of Bonn  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6805-0472","authenticated-orcid":false,"given":"Andras","family":"Bilkei\u2010Gorzo","sequence":"additional","affiliation":[{"name":"Institute of Molecular Psychiatry, Medical Faculty University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6618-6264","authenticated-orcid":false,"given":"Andreas","family":"Zimmer","sequence":"additional","affiliation":[{"name":"Institute of Molecular Psychiatry, Medical Faculty University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9409-2134","authenticated-orcid":false,"given":"Este","family":"Leidmaa","sequence":"additional","affiliation":[{"name":"Institute of Molecular Psychiatry, Medical Faculty University of Bonn  Bonn Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-3-16"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.13044"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200305129"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/celldisc.2017.5"},{"issue":"2","key":"e_1_2_8_6_1","first-page":"175","article-title":"Marijuana, the endocannabinoid system and the female reproductive system","volume":"89","author":"Brents L. 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