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Dp71 shows major expression in perivascular macroglial endfeet, suggesting that dysfunctional glial mechanisms contribute to cognitive impairments. In the present study, we investigated the molecular alterations induced by a selective loss of Dp71 in mice, using semi\u2010quantitative immunogold analyses in electron microscopy and immunofluorescence confocal analyses in brain sections and purified gliovascular units. In macroglial pericapillary endfeet of the cerebellum and hippocampus, we found a drastic reduction (70%) of the polarized distribution of aquaporin\u20104 (AQP4) channels, a 50% reduction of \u03b2\u2010dystroglycan, and a complete loss of \u03b11\u2010syntrophin. Interestingly, in the hippocampus and cortex, these effects were not homogeneous: AQP4 and AQP4ex isoforms were mostly lost around capillaries but preserved in large vessels corresponding to pial arteries, penetrating cortical arterioles, and arterioles of the hippocampal fissure, indicating the presence of Dp71\u2010independent pools of AQP4 in these vascular structures. In conclusion, the depletion of Dp71 strongly alters the distribution of AQP4 selectively in macroglial perivascular endfeet surrounding capillaries. This effect likely affects water homeostasis and blood\u2013brain barrier functions and may thus contribute to the synaptic and cognitive defects associated with Dp71 deficiency.<\/jats:p><\/jats:sec>","DOI":"10.1002\/glia.23941","type":"journal-article","created":{"date-parts":[[2020,11,28]],"date-time":"2020-11-28T15:05:22Z","timestamp":1606575922000},"page":"954-970","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Dp71 contribution to the molecular scaffold anchoring aquaporine\u20104 channels in brain macroglial cells"],"prefix":"10.1002","volume":"69","author":[{"given":"Mehdi","family":"Belmaati Cherkaoui","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, CNRS, Institut des Neurosciences Paris Saclay  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oph\u00e9lie","family":"Vacca","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, CNRS, Institut des Neurosciences Paris Saclay  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Izabelle","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, CNRS, Institut des Neurosciences Paris Saclay  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne\u2010C\u00e9cile","family":"Boulay","sequence":"additional","affiliation":[{"name":"Physiology and Physiopathology of the Gliovascular Unit Research Group, Center for Interdisciplinary Research in Biology (CIRB), Coll\u00e8ge de France, Unit\u00e9 Mixte de Recherche 7241CNRS, Unit\u00e9 1050 INSERM PSL Research University  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Boulogne","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris\u2010Sud Universit\u00e9 Paris\u2010Saclay  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Gillet","sequence":"additional","affiliation":[{"name":"Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris\u2010Sud Universit\u00e9 Paris\u2010Saclay  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Vianney","family":"Barnier","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, CNRS, Institut des Neurosciences Paris Saclay  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alvaro","family":"Rendon","sequence":"additional","affiliation":[{"name":"UPMC Universit\u00e9 Paris 06, INSERM, CNRS, Institut de la Vision Sorbonne Universit\u00e9s  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5312-8476","authenticated-orcid":false,"given":"Martine","family":"Cohen\u2010Salmon","sequence":"additional","affiliation":[{"name":"Physiology and Physiopathology of the Gliovascular Unit Research Group, Center for Interdisciplinary Research in Biology (CIRB), Coll\u00e8ge de France, Unit\u00e9 Mixte de Recherche 7241CNRS, Unit\u00e9 1050 INSERM PSL Research University  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8783-8185","authenticated-orcid":false,"given":"Cyrille","family":"Vaillend","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris\u2010Saclay, CNRS, Institut des Neurosciences Paris Saclay  Gif\u2010sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-018-1812-4"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200106158"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2004.08.049"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2010.04.071"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0437946100"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2336064100"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-017-0405-x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-8966(99)00105-4"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.61.2.11"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-12-0066"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22198"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-015-1500-6"},{"key":"e_1_2_8_14_1","first-page":"e53208","article-title":"Purification of mouse brain vessels","volume":"105","author":"Boulay A. 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