{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T23:36:01Z","timestamp":1790638561997,"version":"4.1.0"},"reference-count":62,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T00:00:00Z","timestamp":1636588800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009670","name":"National Alliance for Research on Schizophrenia and Depression","doi-asserted-by":"publisher","award":["25248"],"award-info":[{"award-number":["25248"]}],"id":[{"id":"10.13039\/100009670","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30CA54174"],"award-info":[{"award-number":["P30CA54174"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["R01NS112389"],"award-info":[{"award-number":["R01NS112389"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["P01AG19316"],"award-info":[{"award-number":["P01AG19316"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01AG060148"],"award-info":[{"award-number":["R01AG060148"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100013030","name":"William and Ella Owens Medical Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100013030","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008635","name":"University of Texas Health Science Center at San Antonio","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008635","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007130","name":"University of Texas System","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007130","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Glia"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The classical complement cascade mediates synapse elimination in the visual thalamus during early brain development. However, whether the primary visual cortex also undergoes complement\u2010mediated synapse elimination during early visual system development remains unknown. Here, we examined microglia\u2010mediated synapse elimination in the visual thalamus and the primary visual cortex of early postnatal C1q and SRPX2 knockout mice. In the lateral geniculate nucleus, deletion of C1q caused a persistent decrease in synapse elimination and microglial synapse engulfment, while deletion of SRPX2 caused a transient increase in the same readouts. In the C1q\u2010SRPX2 double knockout mice, the C1q knockout phenotypes were dominant over the SRPX2 knockout phenotypes, a result which is consistent with SRPX2 being an inhibitor of C1q. We found that genetic deletion of either C1q or SRPX2 did not affect synapse elimination or microglial engulfment of synapses in layer 4 of the primary visual cortex in early brain development. Together, these results show that the classical complement pathway regulates microglia\u2010mediated synapse elimination in the visual thalamus but not the visual cortex during early development of the central nervous system.<\/jats:p>","DOI":"10.1002\/glia.24114","type":"journal-article","created":{"date-parts":[[2021,11,11]],"date-time":"2021-11-11T15:51:13Z","timestamp":1636645873000},"page":"451-465","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["C1q and <scp>SRPX2<\/scp> regulate microglia mediated synapse elimination during early development in the visual thalamus but not the visual cortex"],"prefix":"10.1002","volume":"70","author":[{"given":"Qifei","family":"Cong","sequence":"first","affiliation":[{"name":"Department of Pharmacology University of Texas Health Science Center at San Antonio  San Antonio Texas USA"},{"name":"Institutes of Neuroscience Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Breeanne M.","family":"Soteros","sequence":"additional","affiliation":[{"name":"Department of Pharmacology University of Texas Health Science Center at San Antonio  San Antonio Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anran","family":"Huo","sequence":"additional","affiliation":[{"name":"Institutes of Neuroscience Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurology The Second Affiliated Hospital of Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea J.","family":"Tenner","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Biochemistry University of California  Irvine California USA"},{"name":"Department of Neurobiology and Behavior University of California  Irvine California USA"},{"name":"Institute for Memory Impairments and Neurological Disorders University of California  Irvine California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9333-0065","authenticated-orcid":false,"given":"Gek Ming","family":"Sia","sequence":"additional","affiliation":[{"name":"Department of Pharmacology University of Texas Health Science Center at San Antonio  San Antonio Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,11,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.09.056"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2014.08.008"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3932-10.2011"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3560"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0913449107"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12776"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-0672-0"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn2075"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn.2015.3"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22301-1"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1110189"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2018.04.016"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-017-0814-9"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01276"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-019-0419-y"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1110359"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100616-060509"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1608769114"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-020-02011-z"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad8373"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1167-12.2012"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.31.060407.125533"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(79)90349-4"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/483960"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1109557"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.09.017"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.125"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2019104136"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23192"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.04.001"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/epi.12195"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1202529"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020107121"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2741"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncir.2013.00097"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3952506"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01381"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-cellbio-100913-013038"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4419-5635-4_6"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2012.03.026"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-020-02024-8"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2679-16.2017"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature16549"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/mp.2016.220"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.051508.135302"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaf6295"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0199399"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-0830"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.10.036"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202103052"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2003.11.019"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1376"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000527"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aal3589"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18283"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaz2288"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1011-19.2019"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2019.12.004"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-016-0091-6"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-neuro-070918-050306"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-00763-8"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3641"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.24114","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/glia.24114","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.24114","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T10:41:22Z","timestamp":1693219282000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.24114"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,11]]},"references-count":62,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1002\/glia.24114"],"URL":"https:\/\/doi.org\/10.1002\/glia.24114","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,11]]},"assertion":[{"value":"2021-07-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}