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We present single\u2010cell RNA sequencing and immunohistofluorescence analyses of pituitary cells of adult female rats with a focus on the transcriptomic profiles of nonhormonal cell types. Samples obtained from whole pituitaries and separated anterior and posterior lobe cells contained all expected pituitary resident cell types and lobe\u2010specific vascular cell subpopulations. FSCs and pituicytes expressed S100B, ALDOC, EAAT1, ALDH1A1, and VIM genes and proteins, as well as other astroglial marker genes, some common and some cell type\u2010specific. We also found that the SOX2 gene and protein were expressed in ~15% of pituitary cells, including FSCs, pituicytes, and a fraction of hormone\u2010producing cells, arguing against its stem cell specificity. FSCs comprised two\n                    <jats:italic>Sox2<\/jats:italic>\n                    \u2010expressing subclusters; FS1 contained more cells but lower genetic diversity, while FS2 contained proliferative cells, shared genes with hormone\u2010producing cells, and expressed genes consistent with stem cell niche formation, regulation of cell proliferation and stem cell pluripotency, including the Hippo and Wnt pathways. FS1 cells were randomly distributed in the anterior and intermediate lobes, while FS2 cells were localized exclusively in the marginal zone between the anterior and intermediate lobes. These data indicate the identity of the FSCs as anterior pituitary\u2010specific astroglia, with FS1 cells representing differentiated cells equipped for classical FSC roles and FS2 cells exhibiting additional stem cell\u2010like features.\n                  <\/jats:p>","DOI":"10.1002\/glia.24267","type":"journal-article","created":{"date-parts":[[2022,9,12]],"date-time":"2022-09-12T03:46:15Z","timestamp":1662954375000},"page":"205-228","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["The astroglial and stem cell functions of adult rat folliculostellate cells"],"prefix":"10.1002","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0227-5895","authenticated-orcid":false,"given":"Patrick A.","family":"Fletcher","sequence":"first","affiliation":[{"name":"Laboratory of Biological Modeling, National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6790-5491","authenticated-orcid":false,"given":"Kosara","family":"Smiljanic","sequence":"additional","affiliation":[{"name":"Section on Cellular Signaling Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael M.","family":"Pr\u00e9vide","sequence":"additional","affiliation":[{"name":"Section on Cellular Signaling Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0596-9737","authenticated-orcid":false,"given":"Stephanie","family":"Constantin","sequence":"additional","affiliation":[{"name":"Section on Cellular Signaling Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8372-5941","authenticated-orcid":false,"given":"Arthur S.","family":"Sherman","sequence":"additional","affiliation":[{"name":"Laboratory of Biological Modeling, National Institute of Diabetes, Digestive and Kidney Diseases National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5233-0277","authenticated-orcid":false,"given":"Steven L.","family":"Coon","sequence":"additional","affiliation":[{"name":"Molecular Genomics Core Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7329-7488","authenticated-orcid":false,"given":"Stanko S.","family":"Stojilkovic","sequence":"additional","affiliation":[{"name":"Section on Cellular Signaling Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH)  Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.1996.tb00682.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/eje.1.01949"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1267\/ahc.15020"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2006.03.007"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-016-2540-5"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-14198-8"},{"key":"e_1_2_7_8_1","unstructured":"Bechtold B. 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