{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T21:43:23Z","timestamp":1788385403355,"version":"build-2803163510"},"reference-count":64,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T00:00:00Z","timestamp":1668038400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31871477"],"award-info":[{"award-number":["31871477"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170971"],"award-info":[{"award-number":["32170971"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81528006"],"award-info":[{"award-number":["81528006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["18ZR1403800"],"award-info":[{"award-number":["18ZR1403800"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2014CB965001"],"award-info":[{"award-number":["2014CB965001"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Glia"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In response to central nervous system (CNS) injury, astrocytes go through a series of alterations, referred to as reactive astrogliosis, ranging from changes in gene expression and cell hypertrophy to permanent astrocyte borders around stromal cell scars in CNS lesions. The mechanisms underlying injury\u2010induced reactive astrocytes in the adult CNS have been extensively studied. However, little is known about injury\u2010induced reactive astrocytes during early postnatal development. Astrocytes in the mouse cortex are mainly produced through local proliferation during the first 2\u2009weeks after birth. Here we show that Sox2, a transcription factor critical for stem cells and brain development, is expressed in the early postnatal astrocytes and its expression level was increased in reactive astrocytes after traumatic brain injury (TBI) at postnatal day (P) 7 in the cortex. Using a tamoxifen\u2010induced <jats:italic>hGFAP\u2010CreERT2; Sox2<\/jats:italic><jats:sup><jats:italic>flox\/flox<\/jats:italic><\/jats:sup>; <jats:italic>Rosa\u2010tdT<\/jats:italic> mouse model, we found that specific knockout of Sox2 in astrocytes greatly inhibited the proliferation of reactive astrocytes, the formation of glia limitans borders and subsequently promoted the tissue recovery after postnatal TBI at P7 in the cortex. In addition, we found that injury\u2010induced glia limitans borders were still formed at P2 in the wild\u2010type mouse cortex, and knockout of Sox2 in astrocytes inhibited the reactivity of both astrocytes and microglia. Together, these findings provide evidence that Sox2 is essential for the reactivity of astrocytes in response to the cortical TBI during the early postnatal period and suggest that Sox2\u2010dependent astrocyte reactivity is a potential target for therapeutic treatment after TBI.<\/jats:p>","DOI":"10.1002\/glia.24298","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T09:42:36Z","timestamp":1668073356000},"page":"602-615","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Specific knockout of Sox2 in astrocytes reduces reactive astrocyte formation and promotes recovery after early postnatal traumatic brain injury in mouse cortex"],"prefix":"10.1002","volume":"71","author":[{"given":"Yitong","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wentong","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pifang","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guibo","family":"Qi","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siying","family":"Kang","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5569-4324","authenticated-orcid":false,"given":"Song","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences Fudan University  Shanghai China"},{"name":"State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,11,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/430832"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1098-1136(200010)32:1<1::AID-GLIA10>3.0.CO;2-W"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.224503"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23914"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.24227"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04739-5"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23448"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhx303"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.02.068"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dr.2020.100943"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2004.03813.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500989102"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-020-01800-w"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-020-00783-4"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23687"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1523\/Jneurosci.2532-06.2006"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10959"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02033"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23555"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2004.05.043"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2017.12.003"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2017.11.005"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23269"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-24624-5"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23630"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.14670\/HH-18-338"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23845"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2795-6"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2467"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1523\/Jneurosci.1924-05.2005"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23922"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12974-016-0555-1"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23091"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2019.04.030"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20184540"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11101604"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.22538"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awl165"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.440120304"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13064-020-00151-9"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2004.tb00056.x"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13041-020-00647-9"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7580.2004.00261.x"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.12.007"},{"issue":"1","key":"e_1_2_9_46_1","first-page":"321","article-title":"Roles of sox factors in neural determination: Conserved signaling in evolution?","volume":"45","author":"Sasai Y.","year":"2001","journal-title":"The International Journal of Developmental Biology"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00261"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.032888"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2014.15"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902510103"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1987.tb23675.x"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0079-6123(08)60304-0"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2009.08.002"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00293950"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnana.2018.00008"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aal3589"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-021-03487-8"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20721"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.23955"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2019.0189"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41434-022-00320-x"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-018-1035-7"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1523\/Jneurosci.1291-17.2018"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3655-14.2015"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2020.08.026"}],"container-title":["Glia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.24298","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/glia.24298","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/glia.24298","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T21:57:58Z","timestamp":1692309478000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/glia.24298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,10]]},"references-count":64,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1002\/glia.24298"],"URL":"https:\/\/doi.org\/10.1002\/glia.24298","archive":["Portico"],"relation":{},"ISSN":["0894-1491","1098-1136"],"issn-type":[{"value":"0894-1491","type":"print"},{"value":"1098-1136","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,10]]},"assertion":[{"value":"2021-10-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-11-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}