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While neuronal contribution to FXS has been extensively studied in both animal and human\u2010based models of FXS, the roles of astrocytes, a type of glial cells in the brain, are largely unknown. Here, we generated a human\u2010based FXS model via differentiation of astrocytes from human\u2010induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) and characterized their development, function, and proteomic profiles. We identified shortened cell cycle, enhanced Ca<jats:sup>2+<\/jats:sup>signaling, impaired sterol biosynthesis, and pervasive alterations in the proteome of FXS astrocytes. 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