{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T02:29:28Z","timestamp":1789784968008,"version":"4.0.1"},"reference-count":74,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T00:00:00Z","timestamp":1676505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["20CDA35310104"],"award-info":[{"award-number":["20CDA35310104"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Glia"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Neonatal stroke is common and causes life\u2010long motor and cognitive sequelae. Because neonates with stroke are not diagnosed until days\u2010months after the injury, chronic targets for repair are needed. We evaluated oligodendrocyte maturity and myelination and assessed oligodendrocyte gene expression changes using single cell RNA sequencing (scRNA seq) at chronic timepoints in a mouse model of neonatal arterial ischemic stroke. Mice underwent 60\u2009min of transient right middle cerebral artery occlusion (MCAO) on postnatal day 10 (p10) and received 5\u2010ethynyl\u20102\u2032\u2010deoxyuridine (EdU) on post\u2010MCAO days 3\u20137 to label dividing cells. Animals were sacrificed 14 and 28\u201330\u2009days post\u2010MCAO for immunohistochemistry and electron microscopy. Oligodendrocytes were isolated from striatum 14\u2009days post\u2010MCAO for scRNA seq and differential gene expression analysis. The density of Olig2\n                    <jats:sup>+<\/jats:sup>\n                    EdU\n                    <jats:sup>+<\/jats:sup>\n                    cells was significantly increased in ipsilateral striatum 14\u2009days post\u2010MCAO and the majority of oligodendrocytes were immature. Density of Olig2\n                    <jats:sup>+<\/jats:sup>\n                    EdU\n                    <jats:sup>+<\/jats:sup>\n                    cells declined significantly between 14 and 28\u2009days post\u2010MCAO without a concurrent increase in mature Olig2\n                    <jats:sup>+<\/jats:sup>\n                    EdU\n                    <jats:sup>+<\/jats:sup>\n                    cells. By 28\u2009days post\u2010MCAO there were significantly fewer myelinated axons in ipsilateral striatum. scRNA seq identified a cluster of \u201cdisease associated oligodendrocytes (DOLs)\u201d specific to the ischemic striatum, with increased expression of MHC class I genes. Gene ontology analysis suggested decreased enrichment of pathways involved in myelin production in the reactive cluster. Oligodendrocytes proliferate 3\u20137\u2009days post\u2010MCAO and persist at 14\u2009days, but fail to mature by 28\u2009days. MCAO induces a subset of oligodendrocytes with reactive phenotype, which may be a therapeutic target to promote white matter repair.\n                  <\/jats:p>","DOI":"10.1002\/glia.24349","type":"journal-article","created":{"date-parts":[[2023,2,16]],"date-time":"2023-02-16T04:30:14Z","timestamp":1676521814000},"page":"1429-1450","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Chronic changes in oligodendrocyte\n                    <scp>sub\u2010populations<\/scp>\n                    after middle cerebral artery occlusion in neonatal mice"],"prefix":"10.1002","volume":"71","author":[{"given":"Alexandra P.","family":"Frazier","sequence":"first","affiliation":[{"name":"Department of Anesthesiology University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danae N.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Child Neurology University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine S.","family":"Given","sequence":"additional","affiliation":[{"name":"Department of Developmental and Cell Biology University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevieve","family":"Hunn","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelia M.","family":"Burch","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine R.","family":"Childs","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myriam","family":"Moreno\u2010Garcia","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Corigilano","sequence":"additional","affiliation":[{"name":"Department of Graduate Medical Education University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidia","family":"Quillinan","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1252-0607","authenticated-orcid":false,"given":"Wendy B.","family":"Macklin","sequence":"additional","affiliation":[{"name":"Department of Developmental and Cell Biology University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paco S.","family":"Herson","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The Ohio State University  Columbus Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4696-576X","authenticated-orcid":false,"given":"Andra L.","family":"Dingman","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Child Neurology University of Colorado Anschutz Medical Campus  Aurora Colorado 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