{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T13:18:38Z","timestamp":1787318318490,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T00:00:00Z","timestamp":1694044800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004950","name":"Aston University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","award":["NA150482"],"award-info":[{"award-number":["NA150482"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Glia"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Damage of myelin is a component of many diseases in the central nervous system (CNS). The activation and maturation of the quiescent oligodendrocyte progenitor cells (OPCs) are the crucial cellular processes for CNS remyelination, which is influenced by neuroinflammation in the lesion microenvironment. Endothelial cells derived from human induced pluripotent stem cells (hiPSC\u2010ECs) have shown promise in restoring function in various preclinical animal models. Here we ask whether and whether transplantation of hiPSC\u2010ECs could benefit remyelination in a mouse model of CNS demyelination. Our results show that\n                    <jats:italic>in vitro<\/jats:italic>\n                    , hiPSC\u2010ECs increase OPC proliferation, migration and differentiation via secreted soluble factors including brain\u2010derived neurotrophic factor (BDNF). hiPSC\u2010ECs also promote the survival of oligodendrocyte lineage cells\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Transplantation of hiPSC\u2010ECs into a toxin\u2010induced demyelination lesion in mouse corpus callosum (CC) leads to increased density of oligodendrocyte lineage cells and level of myelin in demyelinated area, correlated with a decreased neuroinflammation and an increased proportion of pro\u2010regenerative M2 phenotype in microglia\/macrophages. The hiPSC\u2010EC\u2010exposed oligodendrocyte lineage cells showed significant increase in the level of phosphorylated S6 ribosomal protein (pS6) both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    , indicating an involvement of mTORC1 pathway. These results suggest that hiPSC\u2010ECs may benefit myelin protection and regeneration which providing a potential source of cell therapy for a wide range of diseases and injuries associated with myelin damage.\n                  <\/jats:p>","DOI":"10.1002\/glia.24466","type":"journal-article","created":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T05:27:56Z","timestamp":1694064476000},"page":"133-155","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Human\n                    <scp>iPSC<\/scp>\n                    \u2010derived endothelial cells promote\n                    <scp>CNS<\/scp>\n                    remyelination via\n                    <scp>BDNF<\/scp>\n                    and\n                    <scp>mTORC1<\/scp>\n                    pathway"],"prefix":"10.1002","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8628-8954","authenticated-orcid":false,"given":"Dan","family":"Ma","sequence":"first","affiliation":[{"name":"Wellcome Trust\u2010Medical Research Council Cambridge Stem Cell Institute and Department of Clinical Neurosciences University of Cambridge  Cambridge UK"},{"name":"Translational Medicine Research Group (TMRG), Aston Medical School Aston University  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Wellcome Trust\u2010Medical Research Council Cambridge Stem Cell Institute and Department of Clinical Neurosciences University of Cambridge  Cambridge UK"},{"name":"Molecular Pharmacology Laboratory, Institute of Molecular Medicine Peking University  Beijing China"},{"name":"ALLIFE Medical Science and Technology Co. 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Ltd  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lida","family":"Wu","sequence":"additional","affiliation":[{"name":"Translational Medicine Research Group (TMRG), Aston Medical School Aston University  Birmingham UK"},{"name":"Molecular Pharmacology Laboratory, Institute of Molecular Medicine Peking University  Beijing China"},{"name":"ALLIFE Medical Science and Technology Co. 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